FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26329512
Sequences flagged as poor quality0
Sequence length35-101
%GC72

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG13190625.009823197634654No Hit
TTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG10415333.9557626438347966No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG7671042.913475950484764No Hit
GGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG5898632.2403111762952537No Hit
GTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG5209121.9784339337546402No Hit
GTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG5097301.9359644797062703No Hit
TGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG4903421.8623284776413631No Hit
AGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG4748701.8035655199382352No Hit
TGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG4486621.7040270248837122No Hit
TTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG4454501.6918277862498932No Hit
ATTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG3730161.4167220417909758No Hit
AGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2794911.0615122680587472No Hit
TATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2631700.999524791800167No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2382370.9048287716080723No Hit
ATGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2367950.8993520274891537No Hit
AATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2304160.8751244610990132No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2129930.8089515673514952No Hit
GAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1966400.7468425544689169No Hit
AAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1947450.7396453075165237No Hit
TAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1620520.6154766560048663No Hit
CGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1608010.6107253336104368No Hit
ACGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1482020.562874085930647No Hit
CTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1422390.540226495652483No Hit
GATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1336850.5077382368499652No Hit
CAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1136040.43147020727159696No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1123690.4267796531891666No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1061240.40306102141201855No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC975270.3704094477710031No Hit
TTTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG948280.36015859314065524No Hit
GGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG885550.33633361681750884No Hit
CTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG841120.3194590161792592No Hit
TCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG828410.31463173339483086No Hit
GCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG735470.2793329401623547No Hit
ACTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG716430.272101511034462No Hit
GGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC566590.21519198684730656No Hit
CGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG556560.2113825732888631No Hit
TCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG556120.2112154604308656No Hit
TTTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC547220.20783522307591573No Hit
CATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG536540.20377893824997592No Hit
CGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC526450.19994673657453282No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC519670.19737167935357103No Hit
TTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC497410.18891728794669646No Hit
TTTGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC492320.18698409602122515No Hit
ATTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG460200.1747848573874062No Hit
CCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG448190.17022343596797387No Hit
GGTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG444460.16880677469449493No Hit
GTTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG418300.15887115568264235No Hit
TGTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG399390.15168910080824893No Hit
GTGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG388830.14767839221630846No Hit
ATTGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC371240.1409976759159076No Hit
GGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC369090.14018110172341972No Hit
AGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC366630.13924678892643358No Hit
AGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG366610.1392391928874337No Hit
GGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC362090.13752248807345915No Hit
TTGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG356010.13521329221749342No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC355550.13503858332049604No Hit
GTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC346740.13169252814104568No Hit
TGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG330250.12542959398563863No Hit
GTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC328470.12475354651464865No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA320510.12173032299269353No Hit
AGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC319830.12147205766669736No Hit
TAACCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCG316960.12038202607021352No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC314400.11940973307822797No Hit
CGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG312840.11881724203623674No Hit
TGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC309890.11769682628375339No Hit
TGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC295410.11219729404783499No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC289570.10997925065986791No Hit
AAACCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCG288270.10948550812487523No Hit
TGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC286020.10863095373738793No Hit
GTTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC284960.1082283636703939No Hit
TTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC284810.10817139337789473No Hit
TTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT284060.10788654191539895No Hit
TATGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG283220.1075675082774037No Hit
GGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG282480.10728645483440787No Hit
AATGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG279870.10629517174492258No Hit
AGTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG274500.10425563527345284No Hit
TGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC274180.10413409864945464No Hit
GTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC267350.10154005133099316No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC267330.10153245529199327No Hit
GGTGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC264930.10062093061200678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)