FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23532915
Sequences flagged as poor quality0
Sequence length35-101
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG364994715.50996551001013No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC6920582.940808650352071No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG5822032.4739944031582994No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG4818552.0475788910978516No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG3383561.4377989297118525No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2818631.1977394215718706No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2128920.904656307983945No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC2018740.8578367788265925No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2003980.851564712658844No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1722420.7319195263315234No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG1411370.5997429557706726No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1408310.5984426493700419No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG1351420.5742679986733475No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG1152640.48979907504021497No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT1051030.446621253678093No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT998380.42424833472606344No Hit
CGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG946860.4023555942814564No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG923860.392582049440114No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG871090.3701581380802166No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG845570.3593137526736488No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG752730.31986262645320396No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC742490.31551127431514536No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC673530.2862076372604074No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA668050.2838789839677745No Hit
TGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG653200.2775686734941251No Hit
TGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG581630.2471559515682609No Hit
TAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG568550.24159777911066266No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTACCT511710.21744437525057989No Hit
TTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC502170.21339047882508394No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC499670.21232813699450323No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG495970.2107558710852438No Hit
TGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA494170.2099909849672257No Hit
GAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGG474790.20175571109656412No Hit
AAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG470660.2000007223924448No Hit
GTTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG451070.19167621180801445No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC448480.19057562567153283No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG429650.182574067003599No Hit
TAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG419020.17805698953996987No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC413400.17566884510482444No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT408240.17347617156650588No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC404350.17182316767812233No Hit
CAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG401590.17065034229716122No Hit
GATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG398730.1694350232429769No Hit
AGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA391710.16645196738270632No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT379330.1611912506376707No Hit
GTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC375330.15949150370874157No Hit
TGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG368860.1567421630511987No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG365670.15538661487537775No Hit
ATGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG342020.14533686115808434No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG341050.14492467252781902No Hit
GGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG334630.1421965787068878No Hit
ATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC325510.1383211557089294No Hit
CTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC323210.13734380122479514No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG314810.133774332674044No Hit
GGGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG308680.13116947050546013No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG304640.1294527261072417No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC300480.12768498930115543No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG298580.12687760950991409No Hit
GGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGG286360.12168488264203564No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG284150.12074577246380229No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC282910.12021885091583427No Hit
CAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG282040.11984915595879218No Hit
TTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG280620.11924574579902235No Hit
AAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG280030.1189950331270053No Hit
GGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAA279580.11880381159750078No Hit
GGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG276140.11734202923862173No Hit
GTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG275400.11702757605676986No Hit
GGTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG273680.11629668487733033No Hit
TTGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG272970.1159949797974454No Hit
GTGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG271780.11548930508608898No Hit
GTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG271090.11519609874084873No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG270810.11507711645582369No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT269670.11459268858107889No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA259950.11046230354378112No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC257500.10942120854981204No Hit
GTGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG252010.10708830588985682No Hit
GGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGG251980.10707555778788987No Hit
GAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG250400.10640415775096286No Hit
GTGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG248300.10551179061327506No Hit
CGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG248150.10544805010344023No Hit
GGTGGCGACGACGCGGGGGACAACGGGGCCCCGCGGGGAAGAGGGGAGGG245950.1045131892925292No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA243910.10364631835877536No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC236550.10051878400954578No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG235740.10017458525643763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)