FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16103534
Sequences flagged as poor quality0
Sequence length35-101
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG6727054.177374978684803No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC1144980.7110116326018873No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1114800.6922704047446977No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG978590.6076864867053406No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC970610.602731052699364No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG785880.4880171023329413No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG714690.4438094147533082No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG644360.4001357714399833No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT625060.3881508245332981No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG574370.35667326190636167No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG513350.31878095826667613No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA481210.2988226062676677No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT420750.26127805238278756No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG405480.251795661747291No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG362820.22530458221158164No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG357690.2221189460648824No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC322950.20054604163284906No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC303570.18851141618976308No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG293570.1823015991396671No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG262570.16305116628436964No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTACCT242130.15035830023397348No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC233040.14471357653543626No Hit
TGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG221550.13757849674487602No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG217900.13531191352159097No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG213820.13277830816515182No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC182830.11353408512690445No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG171750.10665360783539812No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC168870.10486518052497049No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG166050.10311401211684343No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC165020.10247440096068355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)