FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9922786
Sequences flagged as poor quality0
Sequence length35-76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC6689236.741282135884015No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC2396372.4150173146936758No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1843121.8574622086982426No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1780501.7943549321732828No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1714471.7278111207880527No Hit
GGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC1479321.4908313048371697No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC1468811.4802395214408535No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC1366231.3768612968172447No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1181381.1905728895090553No Hit
CGGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT1121921.1306502024733778No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1090091.098572517839244No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC957650.965101938104883No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA892110.8990519396467886No Hit
CGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC733890.7396007532561923No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC728960.734632390540318No Hit
GGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC697830.7032601529449491No Hit
CGGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC625080.629944049987574No Hit
GGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA579770.5842814709497917No Hit
CGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC523870.5279464859969771No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC507440.5113886362156758No Hit
GGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC488380.49218032113158544No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG478050.48176993840238014No Hit
CGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA444700.4481604259126418No Hit
GCCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC424430.4277326952329719No Hit
GGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG422290.4255760428573185No Hit
CGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC422000.42528378622697294No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC419530.4227945659616161No Hit
GGGGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCG409010.4121927047504602No Hit
CGCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA378320.3812638910080294No Hit
CGGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCG365640.3684852217915412No Hit
CGGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGA357530.3603121139567053No Hit
GGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGG354940.35770195991327436No Hit
GCGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC347500.350204065672685No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC345800.3484908371499698No Hit
CGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG332310.33489586493148193No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT331140.33371676059526023No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC327950.3305019376614592No Hit
CGGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCC303220.3055795015633714No Hit
GCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC298350.30067160573653406No Hit
GGCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA292970.2952497413528822No Hit
GGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC285030.28724795637031775No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG282800.28500060366110885No Hit
CGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGG276600.2787523584606178No Hit
GGGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT268410.2704986281070659No Hit
GGGAGAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGG267160.2692389012521282No Hit
GCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC259360.26137820567731684No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG245490.2474002764949279No Hit
GGGGAAGGGCCCCGCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCA239580.2414442879247824No Hit
GCGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA237090.23893491202974648No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA226440.22820203922567714No Hit
GCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC214200.215866793862127No Hit
CGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC213750.21541329219434946No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC209220.21084804207205515No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT205320.20691769428464948No Hit
GGCCCCGTCGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTCCGGG204290.20587967935618082No Hit
CGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG204250.2058393680968228No Hit
GCCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA198280.19982291263764027No Hit
CGGGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCG190500.19198237269250792No Hit
GCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC188160.18962416402006452No Hit
CGCCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC187660.18912027327808945No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT187260.18871716068450936No Hit
CCGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA186980.18843498186900334No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG181820.18323482941182043No Hit
CGCCCCGTCGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTCCGGG176680.17805483258431654No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT176440.1778129650281685No Hit
CCCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA176170.17754086402750194No Hit
GCCCCCGTCGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTCCGGG168560.16987164693464113No Hit
CCGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC163050.1643187709580757No Hit
CGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA162430.1636939464380266No Hit
GCGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA162350.16361332391931055No Hit
GCCCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC159140.1603783453558305No Hit
CGGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCG158070.15930001916800382No Hit
GGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGG157820.1590480737970163No Hit
CGGAGAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGG151160.15233624911390814No Hit
CGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC149960.15112691133316794No Hit
CGGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCG149650.15081449907314337No Hit
CCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC149350.1505121646279583No Hit
GGGGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCCCCC148790.1499478069969462No Hit
GCGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC143400.1445158647984548No Hit
GGGAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGT141980.14308481509124554No Hit
GCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC141420.14252045746023345No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCG136170.13722960466949505No Hit
GGGGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTC135810.13686680333527299No Hit
GCGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC132110.13313801184465734No Hit
CGGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAA131030.13204960784199113No Hit
CGGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGA130690.1317069621374481No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT126490.12747427990485735No Hit
CCCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC123000.12395712252587125No Hit
GGCGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC122620.1235741655619702No Hit
GGCCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC121740.12268731785609405No Hit
GCGGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCG120860.1218004701502179No Hit
GGGGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC118860.11978490718231755No Hit
GCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGGA118670.11959342870036703No Hit
GGGGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCG116130.11703366373113358No Hit
GGGCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG116020.11692280776789907No Hit
GGGGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG115380.11627782761817095No Hit
CCGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT114170.11505841202259125No Hit
GGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAACG110510.1113699317913336No Hit
GGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGACG110420.1112792314577781No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT106720.10755043996716245No Hit
GGGGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCAC105890.1067139813354838No Hit
GGGGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGC105480.10630079092706424No Hit
CGGGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTC104690.10550464355474361No Hit
GGGGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCC104290.10510153096116354No Hit
GGGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGT102820.10362009217975676No Hit
CGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTC102490.10328752429005321No Hit
CGGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGA100240.10102001595116532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)