FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10190467
Sequences flagged as poor quality0
Sequence length35-76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC7761757.616677429994131No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2078192.039347166327117No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1970541.9337092205882225No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1938521.9022876969230162No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1816831.7828721686650866No Hit
CGGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT1816681.7827249722706526No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1600701.5707817904714279No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC1243071.2198361468615717No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA1158971.1373080350488354No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1151711.1301837295582233No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC988110.9696415286953974No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC908530.8915489349016096No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT883730.8672124643551665No Hit
CGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG871080.854798901757888No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG829530.8140255004996335No Hit
GGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC818980.8036726874244331No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT811340.7961754844012547No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC765720.7514081543073542No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT640110.6281458936082124No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC633960.6221108414364132No Hit
CGGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT616720.6051930691694503No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT529630.5197308425609936No Hit
CGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC499360.49002661016418575No Hit
GGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC477830.4688990210164068No Hit
GGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG471240.4624321927542673No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC469120.46035181704626493No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC458740.450165826551423No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT457210.4486644233281949No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC432550.4244653360832237No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC429990.4219531842848812No Hit
GCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT427680.41968635981059554No Hit
CGGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC427000.4190190694891608No Hit
GGGGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTG421720.41383775640507936No Hit
CGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC346940.3404554472331837No Hit
CGGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGA345030.3385811464773891No Hit
GGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA335350.3290820724899065No Hit
GCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC321050.3150493495538526No Hit
CGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC318840.3128806560091898No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG314790.30890635335946826No Hit
CCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT298090.2925184881124683No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG294140.2886423163923694No Hit
CGGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCG289270.2838633401197413No Hit
GGGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT286650.2812923097636252No Hit
CGGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT258780.2539432196777635No Hit
GGCGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC240510.23601469883568635No Hit
CGGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCC232690.22834086013918697No Hit
CGCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA229250.22496515616016421No Hit
CGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA224620.22042169411863066No Hit
GGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGG223840.21965627286757317No Hit
GGGGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCG221770.2176249626243822No Hit
GGGCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG221040.21690860683813606No Hit
GGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC220180.21606468084338037No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC213490.20949972165161812No Hit
GGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG203890.20007915240783372No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC202560.19877401104385106No Hit
GGGGGGCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC202090.19831279567462412No Hit
CCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC200350.19660531749918822No Hit
GCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGGA199340.19561419510999842No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC195930.19226793040986248No Hit
GGGGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGC191860.1882740015742164No Hit
CGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC191530.1879501695064613No Hit
CGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA190930.18736138392872476No Hit
GCGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA190300.1867431590721014No Hit
CGGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGA184970.1815127805232086No Hit
CGGGCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC181200.17781324447643077No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG180690.17731277673535473No Hit
GCCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC180330.1769595053887128No Hit
CGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG180060.17669455187873137No Hit
CGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGG171200.16800015151415534No Hit
GGCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA171190.16799033842119307No Hit
CGGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGG169170.16600809364281344No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCG167490.16435949402515115No Hit
GGGGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCG166650.16353519421632004No Hit
GCGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC162440.15940388207920206No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG162270.15923705949884337No Hit
CGCGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC160800.1577945348333889No Hit
GCCGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC160750.1577454693685775No Hit
GGGGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG158580.15561602819576373No Hit
GGGGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCAC156380.15345714774406316No Hit
GGGGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG155370.15246602535487333No Hit
GGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG153050.15018938778762544No Hit
CGGCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG151780.14894312498141646No Hit
GGCGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC145930.14320246559848532No Hit
CCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT144180.14148517433008714No Hit
GCGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA142170.13951274264466978No Hit
GCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC138650.1360585339219488No Hit
GGGAGAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGG133980.13147581950856618No Hit
CGGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCG129850.12742301211514645No Hit
CCGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT126460.12409637360093508No Hit
GGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC125630.12328188688506621No Hit
GCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC123970.1216529134533285No Hit
CGGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAA123000.12070104343598778No Hit
TTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122850.12055384704155363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121510.11923889258460872No Hit
CGGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGA119690.1174529096654746No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAACGGGACGGG118750.11653047892702072No Hit
GGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC118550.1163342170677752No Hit
CGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG117400.11520571137711352No Hit
GCCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA116470.11429309373162191No Hit
GGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCC116240.11406739259348958No Hit
GGGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGT115440.11328234515650755No Hit
GCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC115270.11311552257614887No Hit
CGGGGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT113970.11183982049105307No Hit
CCGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA112810.1107015017074291No Hit
CGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG111360.10927860322789916No Hit
GGGCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT110290.10822860228093571No Hit
CGGGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTG108930.10689402163806624No Hit
GCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC108430.10640336698995248No Hit
GGCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG104230.1022818679457968No Hit
CGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTC102580.10066270760702134No Hit
CCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGGA102210.10029962316741715No Hit
CCCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA102200.1002898100744549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)