FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9473949
Sequences flagged as poor quality0
Sequence length35-76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG2729892.8814700184685393No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA2345102.475314148302888No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1842011.9442895459960783No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1752081.8493660879956182No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA1579121.6668023017645546No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG1511451.5953748537172832No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1204491.2713705763035035No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG916200.9670729703104798No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC712470.7520306474100715No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG700100.7389737901270104No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG649220.6852686245197225No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG640290.6758427768610533No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC615140.6492962966129542No Hit
ACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA535390.5651180938381661No Hit
ATCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG510760.53912048713794No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG500770.5285757818624526No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG483590.510441844261564No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG478220.5047736693537194No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG468080.49407063517019145No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC441940.46647918412902584No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG432570.45658890500677174No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA427390.4511212800491115No Hit
GCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA425030.44863023856261No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG408880.43158349279693187No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC402890.4252608917358538No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGGCGATGGGGGGGTGGAGCG376550.3974583354839677No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG375760.3966244699016218No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG370400.3909668502543132No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC361190.38124545530063547No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG325710.34379539091882383No Hit
ACCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG314950.33243793058206245No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG306520.3235398459501946No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG287140.30308375103138085No Hit
GCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA287030.30296764316548463No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC286610.30252432222297165No Hit
GTTCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGGCGATGGGGGGGTGGAGCG281700.29734168929978405No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA276810.29218016689766857No Hit
TCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA270410.28542480015461347No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG269070.28401039524278626No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT263960.2786166571088783No Hit
TCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG261920.27646338395952946No Hit
CCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG246810.2605143852895978No Hit
ACCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA242410.25587007065374745No Hit
GTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG242160.25560618914034683No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG238400.251637411178802No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC237200.25037077991447915No Hit
TCGCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA233340.24629644934757405No Hit
CGCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG229650.24240155820978138No Hit
CGTAGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG225390.2379050172214353No Hit
CGGCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG210440.2221249027200801No Hit
CGCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG207710.21924331659374566No Hit
CCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG205490.21690004875474841No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG194530.20533148320726657No Hit
TCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG194310.20509926747547405No Hit
CATCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG188360.19881888745654003No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTACCT187530.19794280083205007No Hit
GCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG186330.19667616956772724No Hit
CTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG182240.19235906800849362No Hit
TACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG179210.18916082406607845No Hit
CCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA177580.18744031659870664No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG176610.18641645632671233No Hit
CCGCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA169550.1789644423882797No Hit
TCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA168900.17827835045343815No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGC166580.1758295300090807No Hit
CCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG166550.17579786422747262No Hit
GCCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG164630.1737712542045561No Hit
TTCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA155420.16404985925087837No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154250.1628148937681636No Hit
CCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG153260.1617699229750973No Hit
ACCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG152040.16048218118970242No Hit
CGCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC151320.1597222024311087No Hit
CGTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC149310.157600595063368No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG147070.15523621670329868No Hit
AGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG141620.14948359971116584No Hit
TGCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG140130.14791086589129834No Hit
CTGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG139400.14714033187216863No Hit
TACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGG132240.13958276532837574No Hit
TACCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA131930.139255552251759No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC131760.1390761128226466No Hit
CCCCATACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG131250.1385377945353094No Hit
CACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC130790.13805225255065232No Hit
TCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC129300.13647951873078482No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA129020.13618397143577615No Hit
TCTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA127870.13497011647413343No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC127570.13465345865805273No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG125710.13269018019835235No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG121740.12849974176555098No Hit
GTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC121430.12817252868893425No Hit
CCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG121360.12809864186518208No Hit
CAGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG120300.1269797842483636No Hit
CCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCG118010.1245626295856142No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG117390.12390820343238074No Hit
CGTTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC115760.1221876959650089No Hit
CCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA114400.12075218053210968No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT112150.11837724691150438No Hit
CTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG111820.1180289233138156No Hit
CCCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA110590.1167306262678847No Hit
TGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC109150.1152106687506973No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG108320.11433458212620735No Hit
CCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG108110.11411292165495086No Hit
AGAGGCGATGGGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCC106840.11277240356687586No Hit
CTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG106610.11252963257454732No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT106420.11232908262436288No Hit
CTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA105970.11185409590024181No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT103000.10871918352104282No Hit
TCGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG102950.1086664072183627No Hit
TGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG102390.10807531262834538No Hit
CCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTGACT101640.1072836680881436No Hit
CACCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA98750.10423319779323278No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG97980.10342044273195898No Hit
CGCTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG97410.10281879288140564No Hit
GAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG97210.10260768767068515No Hit
TCCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCT97080.10247046928371685No Hit
CCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA96610.10197437203852373No Hit
ATACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGGCGATGGGGGGGTGGAGCG94740.10000053831828734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)