FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10435226
Sequences flagged as poor quality0
Sequence length35-76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC7172716.873555014524841No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2174052.083376057212369No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1953551.872072535851164No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1844651.767714470199304No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC1784191.7097760987639365No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1629361.5614036533564295No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1520211.4568060145510984No Hit
GGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC1510051.4470697615940469No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA1172991.124067653158638No Hit
CGGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT1138861.0913611262468106No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC1014640.9723220177502625No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC851190.8156890900110837No Hit
GGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC771850.7396581540256052No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT762820.7310047717222417No Hit
CGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC751090.7197639993613938No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC738840.7080249148413269No Hit
GGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA669970.6420273025232036No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC658280.6308248618669111No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG655350.6280170645082339No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC574980.550999087130456No Hit
CGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC567470.5438023096001946No Hit
CGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA504520.48347778955625875No Hit
GGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG461910.4426449412787035No Hit
CGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG452270.4334070004808712No Hit
CGCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA443800.4252902620412821No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT422250.40463905621210317No Hit
CGGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC412550.3953436178574379No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC412300.3951040447039671No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT410980.3938390984536415No Hit
GGGGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCG403020.38621108924713277No Hit
CGGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCG402290.38551153563899815No Hit
CGGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGA375530.35986762529148864No Hit
GCGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC363260.34810937491914407No Hit
GGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGG350640.3360157221319404No Hit
GGCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA335880.3218713231510271No Hit
CGGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCC310150.29721445419581716No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC300180.28766027683540346No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG295610.2832808795899581No Hit
GGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC294620.2823321699022139No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT284800.2729217364338827No Hit
GCGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA271770.26043518367498697No Hit
GCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC271150.25984104225437954No Hit
CGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGG265990.2548962523667432No Hit
GCCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC263380.2523951086445085No Hit
CGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC263070.25209803793420477No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG250830.24036853634027666No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT244580.23437920750350782No Hit
CGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG239380.22939608591131616No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC238070.2281407225871294No Hit
GCCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA234920.2251221008533979No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA234230.22446087894981864No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC232320.22263054005730204No Hit
CCGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA231970.22229513764244302No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC224320.21496419914623796No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT223510.21418798212899268No Hit
GGGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT221630.21238639201489265No Hit
CCCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA218460.20934860442888345No Hit
GCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC216960.20791116550805894No Hit
CGCCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC214900.20593708272345995No Hit
GCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT210910.20211349519406674No Hit
GGGGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG208070.19939194417063896No Hit
GCGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA207110.19847198326131124No Hit
CGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA203850.19534794934005262No Hit
CGGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT192300.1842796696497038No Hit
CCGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC192270.1842509208712873No Hit
GGGAGAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGG187160.17935404561434512No Hit
GCCCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC186440.17866407493234934No Hit
CGGGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCG181940.17435175816987578No Hit
GGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG181360.17379594845382362No Hit
GGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC180330.1728089070615241No Hit
GGGGAAGGGCCCCGCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCA178090.17066233160642616No Hit
GCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC175720.1683911781115234No Hit
GGGGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCAC172630.1654300539346249No Hit
GGGAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGT168740.16170229566661998No Hit
GGGGGCCGCCGCCGGGCGCACTTCCACCGTGGCGGTGCGCCGCGACCGGC168190.16117523472898432No Hit
GGGGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG165890.1589711617170534No Hit
GGGGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTG164560.15769663254058897No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG156050.1495415623964445No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC155660.14916782827703012No Hit
GCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC153860.1474429015720407No Hit
CGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC152230.14588088461141138No Hit
GGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGG149940.14368639452561927No Hit
GGGCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG147160.1410223410590245No Hit
GCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGGA147100.14096484350219152No Hit
GCGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC145770.13969031432572712No Hit
GCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC144970.13892368023462068No Hit
CGGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAA144470.1384445339276792No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAACGGGACGGG143770.1377737290979611No Hit
GGGGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCG142330.13639378773396954No Hit
CCGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT142260.13632670725099771No Hit
CCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT141270.13537799756325353No Hit
CGGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCG139830.133998056199262No Hit
GGGGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCACTTC139490.13367223671054176No Hit
GGCCCACCGTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGC138560.13278102457963056No Hit
GGGGGGCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC136520.13082610764730923No Hit
GGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAACG133300.1277404054306059No Hit
GGGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGT132530.127002520117916No Hit
GGGGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCCCCC131280.12580465435056223No Hit
CGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC131240.1257663226460069No Hit
CCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC129100.12371557645229725No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCG127500.12218230827008442No Hit
GGCGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC127030.12173191074155941No Hit
CGGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGA121010.11596298920598365No Hit
GCGGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCG119720.11472679173407457No Hit
CGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG118090.11316477477344525No Hit
CCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC113770.10902495068147063No Hit
CGGCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG112200.10752043127767429No Hit
CGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTC110650.10603507772615561No Hit
CCGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC108010.10350518522550448No Hit
CGGAGAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCCTCGCGTCGGG105230.10084113175890969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)