FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8503507
Sequences flagged as poor quality0
Sequence length35-76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2882223.389448612202001No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2072882.4376765962561096No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1123481.3211960665170264No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG980901.1535240695397793No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC797930.9383540226403059No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG722620.8497905628818793No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA694480.8166983339932571No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG609000.7161751028134627No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC550660.6475681151317921No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC488550.5745276625279429No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG481610.5663663239178847No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG437340.514305450680525No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG426170.5011696938686592No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG378900.4455808644598046No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC377770.44425200096854156No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA362100.42582430989943326No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT324820.38198357454165677No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC292430.3438934077434169No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG278820.3278882465787351No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG267220.3142468160489549No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG266170.31301203138893163No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263670.3100720679126859No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA262370.3085432869050381No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG261630.3076730577160694No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC256940.3021576862346324No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC249920.29390226879333436No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC230420.27097055367861755No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG212200.24954409986373857No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC209920.24686285317340245No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGC208070.2446872802009806No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG206880.24328785758628763No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG204210.24014797659365716No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCA197360.23209247666874386No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC193750.22784716940904498No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG190220.223695940980586No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG189490.22283747164552223No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA187250.2202032643708061No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC176580.20765550025418925No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTACCT175440.2063148769090212No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG175330.20618551851606637No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC173890.20449209955374884No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG171620.20182261271731772No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG169900.19979991784566062No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT167930.197483226626379No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT160770.18906317123041116No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG160660.18893381283745636No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG160240.1884398989734471No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG154760.18199549903351642No Hit
CGCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG152890.1797964063532846No Hit
CGTAGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG146640.17244649766267026No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG145230.17078835826206765No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT145170.17071779913863774No Hit
CGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACC144200.16957709330985438No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCC139090.16356780796440812No Hit
TCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC134910.15865218903212522No Hit
CGGCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG133260.15671181313780302No Hit
GATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCA123650.1454105935351144No Hit
CATCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG123380.14509307747967987No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG121590.14298806363068792No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG114520.13467384691986495No Hit
GCCGCCCCGGAGCCCGCGCCGCGCGCCGACCCCCGACCCGCCCCCCGACG113500.13347434182155668No Hit
TGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG111650.13129876884913483No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG110440.1298758265266319No Hit
CCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG108480.12757089516125525No Hit
GGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC107920.1269123433425762No Hit
CACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGC107110.12595979517627257No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG104990.12346670614841618No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT101640.11952715509024688No Hit
GAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCG101330.1191625996191924No Hit
TACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGG97750.1149525719212085No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG97640.1148232135282537No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG96570.11356490916042052No Hit
AGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG95910.11278875880269164No Hit
GTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCC94090.11064846539198474No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG93180.10957831868663129No Hit
CGCCGCCCCGGAGCCCGCGCCGCGCGCCGACCCCCGACCCGCCCCCCGAC92950.10930784204681669No Hit
AGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG92050.10824945519536822No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA91170.10721458805172972No Hit
GCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGGCGATGGGGGGGTGGAG89720.10550940923550718No Hit
CTGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG88030.10352199392556506No Hit
TGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACC87560.10296928079203085No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT86210.10138170051485816No Hit
CCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTGACT85540.10059379030322431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)