FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8503507
Sequences flagged as poor quality0
Sequence length35-76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG6788707.983412020475787No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC3253663.8262566256486883No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2859033.3621775109963457No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2777453.2662406228394945No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1457601.7141163051903174No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC1362791.602621130317174No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1286171.5125171296971944No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1202771.4144399481296364No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA1018641.197905758177185No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG886881.0429579231251294No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC880371.0353022582329854No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC878621.0332442837996134No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC766040.9008518485373154No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC736670.8663131576183803No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT726160.8539535511642432No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT663590.7803721452807648No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC585650.6887158439453275No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT448930.5279351213564004No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC448740.5277116841322057No Hit
GGGGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTA443150.5211379257993202No Hit
GGGGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTG438770.5159871097889377No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC428760.5042154960300497No Hit
GCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGGA383270.4507199206162822No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC379350.44611005788552893No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT357960.42095573038277034No Hit
CGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG343150.4035393867494906No Hit
GGGGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC337300.3966598722150755No Hit
GGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC329060.38696975259736954No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC325860.38320659934777496No Hit
GCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT312510.3675071943846227No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC291670.34299965884663824No Hit
GGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG277040.32579499258364814No Hit
GGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC273040.32109105102165497No Hit
CCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGGA252950.2974655045265442No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC248090.29175021552872243No Hit
GCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC237830.2796846054222099No Hit
GGGGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC237010.2787202974020013No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT236460.27807350543722725No Hit
CCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT232980.2739810762782932No Hit
GGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG219800.2584815888315256No Hit
GGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG214410.2521430275767398No Hit
GCGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA202820.23851335690086456No Hit
GGGGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGAGGA200750.2360790671425331No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT199290.23436212847240556No Hit
GGGGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTA197190.23189255915235912No Hit
CGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA188530.2217085256706439No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC181350.21326495056686615No Hit
GCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC179910.21157153160454856No Hit
CGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG172060.20234004628913696No Hit
CGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC171670.20188141198684262No Hit
GCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGGAA170900.20097590323615888No Hit
GGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG167670.1971774704248494No Hit
TTTAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161670.19012155808185963No Hit
GCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC160260.18846341868125704No Hit
CCGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG149530.1758450954412103No Hit
CGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG149350.17563341807092064No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG149220.17548053997015584No Hit
CGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC148600.1747514290280469No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC146150.17187026482132609No Hit
CGGGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTA141310.1661784955313143No Hit
CCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC128720.15137283946494076No Hit
CGGGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC127670.15013805480491754No Hit
GGGGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG126150.14835055701136013No Hit
GCGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC125260.14730393001381664No Hit
GGCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG122820.14443452566100082No Hit
CCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT116480.1369787782852416No Hit
CGGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT114990.13522656005339914No Hit
CGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG113730.1337448184613713No Hit
GGGCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT109790.129111436022808No Hit
CCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGGAA108610.12772377326202No Hit
GCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC108210.12725337910582069No Hit
CCGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA105580.12416053752881018No Hit
CGGGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTG103220.12138521200723419No Hit
GGGGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGAT102970.1210912156596096No Hit
CCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC101650.11953891494415186No Hit
GGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAACG101240.11905676093404757No Hit
GGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA99330.11681062883819582No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAACGGGACGGG97800.11501137119073342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95940.11282403836440659No Hit
GGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC95880.11275347924097669No Hit
GCGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC93620.11009575225845054No Hit
CGGGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC93520.10997815371940071No Hit
GGGGGGGTCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGG92650.10895504642966719No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCG89170.10486261727073312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)