FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9185352
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3053413.324216644065464No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC333260.3628167978755741No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG147470.16054910035020975No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC130350.14191072916966058No Hit
TTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121720.13251533528600756No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCG103070.11221126855018729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)