FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9728220
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5422565.5740515736691805No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG219130.22525189603031182No Hit
GGGGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG128590.13218245475534066No Hit
TTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113150.11631110316172948No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCG105730.1086838085487376No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG104690.10761475377818347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)