FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9113307
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1814711.9912749565004229No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT403330.44257260289815764No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382890.4201438621567341No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA363260.3986039315914629No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG309860.34000829775623714No Hit
GTTTCGGTGTAATGGAGATAGGCAGGCGTGTACGGGGTCGAGTCCTCTAT195160.21414838762701618No Hit
ATTGATTTGAGTTGGTGATACTGTTGGGTCAGAACCAGGGCCTGCACCACCGAAATTCTGTCTTTTACAAACTGG159920.17547965848182223No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG147780.1621584788046754No Hit
TCTGGATCTATTGATTTGAGTTGGTGATACTGTTGGGTCAGAACCAGGGCCTGCACCACCGAAATTCTGTCTTTT143970.1579777790872183No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT139880.15348983634590604No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTA133950.1469828680192602No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTCC128270.1407502238210564No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATT109920.1206148327934086No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA107880.11837634790532131No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA106330.11667553830898049No Hit
GGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCATAG103460.11352629731446554No Hit
CGCCCTGGGAAAACCACCTTCGTGATCATGGTATCTCCCCTGCCCCC102940.11295570312730603No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG101800.11170478510161021No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT99660.10935657056214611No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC95180.10444068218046425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)