FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7930808
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2939033.70583930414152No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG412660.5203252934631629No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219060.27621397466689396No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA218940.2760626659982186No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT197930.2495710399243053No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA184420.23253620564260288No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG143410.18082646812279404No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC138400.1745093312055972No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC122250.15414570621303655No Hit
CGCCCTGGGAAAACCACCTTCGTGATCATGGTATCTCCCCTGCCCCC112850.14229319383346564No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG94170.1187394777429992No Hit
GTTTCGGTGTAATGGAGATAGGCAGGCGTGTACGGGGTCGAGTCCTCTAT86880.10954747612097027No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG86180.10866484222036392No Hit
GTCTGTTTCTCTCTCTTTCTGTTTCTCTCTGTCTCTGTCCATCTCTGTCT83150.10484429833631075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)