FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10399411
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5111974.915634164281035No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1355771.3036988344820681No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1128611.0852633865514114No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA649070.6241411172228889No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT559790.5382901012374642No Hit
CGCCCTGGGAAAACCACCTTCGTGATCATGGTATCTCCCCTGCCCCC497570.47845978969385866No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC363720.34975057722019065No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG333110.32031621790887965No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318020.30580578073123565No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT306150.2943916727591592No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA284510.2735828019490719No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATT228180.21941627271006023No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTCC209060.2010306160608519No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG208750.20073252225534693No Hit
TCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204740.19687653464220234No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197540.18995306561111971No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTA194090.18663557003372594No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165250.1589032301925561No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157290.15124895054152585No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151500.14568132752903024No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG143210.1377097222140754No Hit
GGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATT142790.13730585318726224No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131290.1262475345959497No Hit
GGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCATAG128160.12323774875327073No Hit
TTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG127810.12290119123092645No Hit
TGGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCT126480.12162227264601812No Hit
GGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACT126100.12125686733604432No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA121650.11697777883766687No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC119890.11528537529673555No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA116790.1123044372416861No Hit
TGGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTAC116420.11194864786092214No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG114710.11030432396604No Hit
AGCCTCGCCCTGGGAAAACCACCTTCGTGATCATGGTATCTCCCCTGCCCCC108810.10463092573223617No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT106590.10249618944765237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)