FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008087775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008087775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56360928
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCG5710551.0132107831865367TruSeq Adapter, Index 1 (97% over 36bp)
GGGACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTC4608370.8176533218189737No Hit
GGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA4579880.8125984015025444No Hit
GGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT2935650.5208661574912322No Hit
GGCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA2832580.5025786658445368No Hit
GGCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCG2667210.4732374172405394No Hit
GGCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC2466720.4376649014721688No Hit
GGCCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC2460770.43660920558298827No Hit
GGCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGT1895080.33624002784340246No Hit
GGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACAC1721050.30536225379397586No Hit
CGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA1571260.2787853315687066No Hit
GGGAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT1497600.2657159938885322No Hit
GGGGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTA1485800.2636223448982245No Hit
GGCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTC1459210.258904537554811No Hit
GGGCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGA1420530.25204162713573486No Hit
GGCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT1362850.24180758698650243No Hit
GGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC1293300.2294674778953249No Hit
GGGGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGC1279330.22698881040425734No Hit
GGCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCT1210110.21470725251365624No Hit
GGGCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC1152740.2045282150073895No Hit
CGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT1145070.2031673431636896No Hit
GGCCCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTA1114790.1977948269411036No Hit
GGGCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCT1092910.19391270491500778No Hit
GGGCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTC1086790.19282684628613636No Hit
GGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACT1047320.18582376784143795No Hit
GGGCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGT991570.17593216350163715No Hit
GGGCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACT991380.1758984522043356No Hit
GGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATAC968750.17188325926783887No Hit
GGGTCTCCCCCGGATTTTCAAGGGCCAGCGAGATCGGAAGAGCACACGTCT950780.16869488025463314Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATAC949140.16840389853055648No Hit
GGCAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT946290.16789822907103305No Hit
GGGCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCA940890.16694011851614649No Hit
GGGGGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAT908770.16124113499337697TruSeq Adapter, Index 1 (97% over 36bp)
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG892030.15827099227322872No Hit
GGCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACAC890740.15804211030733917No Hit
GGGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC842900.1495539605025666No Hit
GGGAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGA837650.14862246412976024No Hit
GGGCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC824450.14628041610670428No Hit
GGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCC820920.14565409568841733No Hit
GGGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTG802350.1423592599468909No Hit
GGGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAAT757050.13432177695867606No Hit
GGCCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAAT752330.133484317362553No Hit
GGGAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA751780.13338673202825901No Hit
GGCCCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGG718040.12740031533902352No Hit
CGGACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTC709220.12583540143270885No Hit
GGCAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGC702330.12461292333582584No Hit
GGGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGC696160.12351819331292771No Hit
GGGAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAG688690.12219280704533467No Hit
GGGCCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTA685900.12169778325864329No Hit
GGGCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAAT681640.12094194048756614No Hit
GGGAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAA672080.11924572994965592No Hit
GGGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGC655080.11622945598056868No Hit
GGGGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG653490.11594734565051874No Hit
GGCAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA652930.1158479860374194No Hit
GGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAAT641480.11381643680529888No Hit
GGCTCTCCCCCGGATTTTCAAGGGCCAGCGAGATCGGAAGAGCACACGTCT632190.11216813179513298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAG621470.11026610491580266No Hit
GGGCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTT619730.10995738040367256No Hit
GGGGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAA606340.10758162108331502No Hit
GGGCTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTG605150.10737048190547893No Hit
GGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCC598730.10623139491244715No Hit
GGGCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGC592660.10515440767760248No Hit
GGGCAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTT592310.10509230791941539No Hit
GGGGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGCTCG583730.103569976704429No Hit
CGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACAC581220.10312463272428729No Hit
GGCCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGC567550.1006991935973801No Hit
GGGGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTA563690.10001432197851674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)