FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008089122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008089122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28731184
Sequences flagged as poor quality0
Sequence length25-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC2685730.9347787407577773No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1821490.6339766575578647No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1806670.6288184990914402No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT1606700.5592181651824721No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC1503990.5234695514114559No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA1460420.50830484396327No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1320150.45948332654860313No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1291150.44938976409743503No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG1080550.3760896174692975No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC970370.33774104123241144No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC923920.3215739386166613No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA878220.30566787640913096No Hit
CACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGG815550.28385533989827916No Hit
CCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTG809330.28169044477944244No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT808160.28128322174261944No Hit
CCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGC805700.2804270092036583No Hit
CTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGTT768300.2674097941804278No Hit
CACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCT741710.25815504157433955No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG724420.2521371900301777No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC718270.2499966586827748No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC675160.23499205601829704No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC653810.22756110573097163No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA640120.22279624814626506No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC630360.21939924230063054No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG605700.21081623367836147No Hit
CAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATG593930.20671963953869774No Hit
CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT586840.20425193754632598No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT585210.2036846097257948No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC581100.20225410828874996No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC560020.19491713254838366No Hit
GTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTT550120.19147139915988148No Hit
CTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTG546370.19016619711878216No Hit
CACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTC536180.18661952810576826No Hit
CGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAG531780.18508809104421176No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG519510.1808174699657348No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTC499770.173946886421388No Hit
CTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTG468360.16301451412514015No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA461770.1607208390715816No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG452390.15745609369944516No Hit
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT448060.1559490204093225No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC430930.14998685748558083No Hit
CAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCG428600.14917589195071113No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGA415530.14462682777013297No Hit
CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCC411220.14312671555756282No Hit
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG411220.14312671555756282No Hit
GAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCA407780.1419294102185277No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT403210.14033880399777468No Hit
TGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACA397380.13830964989121228No Hit
CCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT393180.13684782360518105No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC389600.13560179072327824No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA389200.13546256917222763No Hit
CTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACC370890.12908970267288672No Hit
CCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGC369660.12866159640340613No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG367390.12787151410119402No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGG364100.1267264168438029No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA350780.1220903391938181No Hit
CTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCA346410.1205693437485904No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG344770.11999853538928296No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT336090.11697742773148508No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG333700.11614557896395777No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT328290.11426260748599848No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC324990.1131140296898311No Hit
CCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAG323040.11243532462845945No Hit
GCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGA320640.11159999532215589No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT318930.11100482319141461No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA315540.10982492054626082No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGAG311550.10843618557453115No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG310640.10811945654589103No Hit
GGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACA307600.10706137275790653No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC306390.10664022756597849No Hit
CAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGG305610.10636874554142983No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA303440.10561346862698036No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG301240.10484775009620209No Hit
CTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAG299040.10408203156542382No Hit
CGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACC295740.10293345376925643No Hit
ACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGG295020.10268285497736535No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC294450.10248446426711826No Hit
GTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGG293590.10218513793235949No Hit
CTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACG288970.10057712901772513No Hit
GTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAA287980.10023255567887492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)