FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54833
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA37206.784235770430215No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA14222.5933288348257437No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9841.7945397844363795No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9561.7434756442288404No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9141.6668794339175315No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8381.528276767639925No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8351.5228056097605458No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8061.46991775025988No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7701.4042638557073295No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7481.3641420312585486No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7131.3003118559991245No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6661.2145970492221836No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6511.1872412598252877No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6351.1580617511352653No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6281.1452957160833805No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6261.1416482774971277No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6151.121587365272737No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6141.1197636459796108No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6141.1197636459796108No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6001.0942315758758412No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5851.066875786478945No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5831.0632283478926923No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5751.0486385935476812No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5661.0322251199095436No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5480.9993981726332682No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5420.98845585687451No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5390.9829846989951306No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA5220.9519814710119818No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5220.9519814710119818No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4990.9100359272700747No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4980.9082122079769482No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4920.8972698922181898No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA4560.8316159976656393No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4300.7841992960443529No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4250.7750806995787208No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4110.7495486294749512No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4020.7331351558368137No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3860.7039556471467912No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3850.7021319278536647No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3690.6729524191636423No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3600.6565389455255046No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3590.6547152262323783No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3050.5562343844035527No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2710.494227928437255No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2160.39392336731530286No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2140.39027592872905004No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1920.35015410428026916No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1690.30820856053836193No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1560.2845002097277187No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1470.2680867360895811No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA1390.25349698174456986No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1340.24437838527893788No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA1330.24255466598581146No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGA1310.23890722739955866No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1280.23343606952017948No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1200.21884631517516823No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1180.21519887658891543No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA1160.21155143800266263No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1100.20060912224390423No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1090.1987854029507778No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1080.19696168365765143No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1080.19696168365765143No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1070.19513796436452502No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1030.1878430871920194No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1030.1878430871920194No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1010.1841956486057666No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1000.1823719293126402No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA990.1805482100195138No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA980.1787244907263874No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA980.1787244907263874No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA960.17507705214013458No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA950.1732533328470082No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA940.17142961355388178No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA920.16778217496762898No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA910.16595845567450257No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA900.16413473638137616No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA900.16413473638137616No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA890.16231101708824977No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA880.16048729779512336No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA860.15683985920887056No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA850.15501613991574417No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA840.15319242062261776No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA840.15319242062261776No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA830.15136870132949137No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA830.15136870132949137No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA830.15136870132949137No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA820.14954498203636496No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA820.14954498203636496No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA820.14954498203636496No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA800.14589754345011216No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA770.14042638557073295No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA740.13495522769135374No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA730.13313150839822735No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA730.13313150839822735No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA720.13130778910510094No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA710.12948406981197455No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA710.12948406981197455No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA710.12948406981197455No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA700.12766035051884814No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA690.12583663122572175No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA680.12401291193259534No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA680.12401291193259534No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA680.12401291193259534No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA670.12218919263946894No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA660.12036547334634254No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA660.12036547334634254No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA660.12036547334634254No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA650.11854175405321614No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA640.11671803476008974No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.11671803476008974No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.11671803476008974No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.11307059617383691No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.11307059617383691No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.11307059617383691No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA610.11124687688071051No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA610.11124687688071051No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.11124687688071051No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGA600.10942315758758411No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA590.10759943829445771No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA590.10759943829445771No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA580.10577571900133131No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA570.10395199970820491No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA550.10030456112195212No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA550.10030456112195212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)