FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43758
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA15153.462224050459345No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA13733.137711961241373No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA7271.6614104849398965No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6141.4031719914072855No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA4941.1289364230540702No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4471.0215274921157274No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4451.0169568993098403No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4300.9826774532656886No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4060.9278303395950455No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA3640.8318478906714201No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3570.8158508158508158No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA3520.8044243338360985No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA3460.7907125554184378No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3320.7587184057772294No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3280.7495772201654555No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA3250.7427213309566251No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3160.7221536633301339No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA3150.7198683669271905No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3100.7084418849124732No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2970.6787330316742082No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2960.6764477352712647No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2920.6673065496594908No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2860.6535947712418301No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2800.6398829928241693No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2780.6353124000182824No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2760.6307418072123955No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2690.6147447323917912No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2670.6101741395859043No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2670.6101741395859043No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2660.6078888431829609No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2640.603318250377074No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2590.5918917683623566No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2570.5873211755564697No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2570.5873211755564697No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2560.5850358791535262No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2560.5850358791535262No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2550.5827505827505828No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2540.5804652863476393No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2540.5804652863476393No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2450.5598976187211481No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2390.5461858403034874No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2360.539329951094657No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.5324740618858266No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2300.5256181726769962No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2300.5256181726769962No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2260.5164769870652224No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2250.5141916906622789No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2190.5004799122446182No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2160.49362402303578773No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2160.49362402303578773No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2150.4913387266328443No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2150.4913387266328443No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2100.4799122446181269No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2080.4753416518122401No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2070.4730563554092966No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2050.4684857626034097No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2050.4684857626034097No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1990.4547739841857489No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1930.44106220576808813No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1930.44106220576808813No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1900.43420631655925773No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1900.43420631655925773No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1880.4296357237533708No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA1870.4273504273504274No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1850.42277983454454043No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1830.41820924173865354No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1820.41592394533571003No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1800.4113533525298231No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.4067827597239362No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.4044974633209927No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.3839297956945016No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.38164449929155814No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.38164449929155814No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.38164449929155814No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1640.37478861008272774No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1620.3702180172768408No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1610.3679327208738974No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1570.3587915352621235No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1540.35193564605329314No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1530.34965034965034963No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1520.3473650532474062No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1490.34050916403857584No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.33822386763563234No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1470.3359385712326889No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1460.3336532748297454No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1420.3245120892179716No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1420.3245120892179716No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1390.3176562000091412No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1370.3130856072032543No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1340.3062297179944239No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1340.3062297179944239No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1320.301659125188537No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1310.2993738287855935No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1310.2993738287855935No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1300.29708853238265004No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1300.29708853238265004No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1280.2925179395767631No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1270.29023264317381964No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1240.28337675396498924No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.2696649755473285No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1170.267379679144385No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1170.267379679144385No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1160.2650943827414416No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA1150.2628090863384981No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1100.2513826043237808No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1080.24681201151789386No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1080.24681201151789386No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1050.23995612230906346No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1050.23995612230906346No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1020.2331002331002331No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA970.2216737510855158No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA960.21938845468257231No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA960.21938845468257231No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA950.21710315827962887No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA950.21710315827962887No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA940.2148178618766854No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA910.20796197266785502No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA880.20110608345902461No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA870.1988207870560812No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.19653549065313772No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA860.19653549065313772No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.19653549065313772No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA850.19425019425019424No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA840.1919648978472508No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA840.1919648978472508No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA830.18967960144430734No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA830.18967960144430734No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA820.18739430504136387No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA810.1851090086384204No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA780.17825311942959002No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.1736825266237031No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.15768545180309887No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA670.15311485899721194No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.14854426619132502No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.14854426619132502No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA630.1439736733854381No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA610.13940308057955117No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA610.13940308057955117No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.13483248777366424No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA560.12797659856483384No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA560.12797659856483384No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA560.12797659856483384No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.12797659856483384No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA560.12797659856483384No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA500.11426482014717308No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA500.11426482014717308No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA490.11197952374422963No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.10969422734128616No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA480.10969422734128616No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA470.1074089309383427No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA470.1074089309383427No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA470.1074089309383427No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA460.10512363453539925No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA460.10512363453539925No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA450.10283833813245577No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA440.10055304172951231No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA440.10055304172951231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)