FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37349
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA13353.5743928886984926No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA7662.050925058234491No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA5481.4672414254732389No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4861.3012396583576535No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3851.0308174248306514No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3390.9076548234223137No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3380.904977375565611No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3370.902299927708908No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3060.8192990441511151No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2940.7871696698706793No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2920.7818147741572733No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA2880.7711049827304614No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2670.7148785777396984No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2660.7122011298829956No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2600.6961364427427775No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2440.6532972770355298No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2290.6131355591849849No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2290.6131355591849849No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2280.6104581113282819No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2220.594393424188064No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2220.594393424188064No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2200.589038528474658No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2190.5863610806179549No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA2180.5836836327612519No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2160.578328737047846No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2110.564941497764331No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA2110.564941497764331No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2080.5569091541942222No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA2060.5515542584808161No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2050.5488768106241131No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA2030.5435219149107071No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1980.5301346756271922No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1970.5274572277704892No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1960.5247797799137862No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1930.5167474363436773No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1900.5087150927735682No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1860.4980053013467563No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1820.4872955099199443No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1780.47658571849313236No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1720.4605210313529144No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1690.4524886877828055No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1680.44981123992610245No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1670.44713379206939946No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1670.44713379206939946No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1660.4444563442126965No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1660.4444563442126965No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1640.43910144849929045No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1630.43642400064258746No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1620.4337465527858845No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1600.42839165707247845No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1600.42839165707247845No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1550.4150044177889636No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1530.40964952207555755No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1520.40697207421885456No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1510.4042946263621516No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1480.3962622827920427No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1470.39358483493533963No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.39090738707863665No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1450.38822993922193366No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1440.3855524913652307No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA1420.3801975956518247No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1420.3801975956518247No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1400.3748426999384187No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1380.3694878042250127No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1380.3694878042250127No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1360.36413290851160673No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1350.36145546065490375No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1350.36145546065490375No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1340.35877801279820076No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA1330.3561005649414978No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA1330.3561005649414978No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1320.3534231170847948No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1310.3507456692280918No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1310.3507456692280918No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1310.3507456692280918No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1290.3453907735146858No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1270.3400358778012798No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1260.3373584299445768No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.3373584299445768No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1260.3373584299445768No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1200.32129374280435885No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.31593884709095293No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1160.3105839513775469No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1140.30522905566414094No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.294519264237329No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1080.289164368523923No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1050.281132024953814No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1050.281132024953814No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1040.2784545770971111No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1030.27577712924040804No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1010.27042223352700206No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1000.2677447856702991No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1000.2677447856702991No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA990.2650673378135961No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA980.2623898899568931No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA980.2623898899568931No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.24900265067337815No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.24900265067337815No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.24632520281667514No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA910.24364775495997215No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA900.24097030710326914No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA900.24097030710326914No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA890.23829285924656618No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA880.2356154113898632No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA870.23293796353316018No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA860.2302605156764572No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.22490561996305122No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.22222817210634824No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA810.21687327639294224No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA800.21419582853623922No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA800.21419582853623922No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA790.21151838067953627No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA770.2061634849661303No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA760.20348603710942728No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA750.20080858925272427No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.19545369353931832No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA730.19545369353931832No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA720.19277624568261534No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.19009879782591235No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.19009879782591235No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA700.18742134996920934No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.18206645425580337No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.18206645425580337No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA670.17938900639910038No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA670.17938900639910038No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.17403411068569438No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA640.17135666282899142No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA630.1686792149722884No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA620.16600176711558542No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA610.16332431925888244No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA600.16064687140217943No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.15796942354547647No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.15529197568877345No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.15529197568877345No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA570.15261452783207047No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA560.14993707997536748No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA560.14993707997536748No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.1472596321186645No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA540.1445821842619615No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA530.1419047364052585No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.1419047364052585No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.13387239283514954No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA490.13119494497844655No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA480.12851749712174354No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA480.12851749712174354No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA480.12851749712174354No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.12316260140833757No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.12316260140833757No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA460.12316260140833757No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA460.12316260140833757No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.1178077056949316No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA430.1151302578382286No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA430.1151302578382286No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA430.1151302578382286No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA430.1151302578382286No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.11245280998152561No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.10977536212482261No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA410.10977536212482261No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA400.10709791426811961No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA400.10709791426811961No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.10709791426811961No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.10709791426811961No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA400.10709791426811961No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.10442046641141664No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA390.10442046641141664No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA390.10442046641141664No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA390.10442046641141664No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA380.10174301855471364No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.10174301855471364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)