FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48315
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA18393.8062713443030116No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA14252.9493945979509473No Hit
TCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA6671.3805236469005484No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA5441.125944323708993No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5331.1031770671634067No Hit
TCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4871.0079685397909552No Hit
GCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4820.9976197868156887No Hit
TCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4790.9914105350305288No Hit
TCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4680.9686432784849425No Hit
ACCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4530.9375970195591431No Hit
ACCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4040.8361792404015316No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3880.8030632308806789No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3860.7989237296905723No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3860.7989237296905723No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3840.7947842285004657No Hit
GCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3680.761668218979613No Hit
TCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3620.7492497154092932No Hit
ACCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3460.7161337058884405No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3450.7140639552933872No Hit
TCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3440.7119942046983339No Hit
GCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3350.6933664493428542No Hit
CCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3280.6788781951774812No Hit
TCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3200.6623201904170547No Hit
TCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3100.6416226844665218No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3100.6416226844665218No Hit
GCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3040.629204180896202No Hit
CCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3040.629204180896202No Hit
TCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3010.6229949291110422No Hit
ACCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2960.6126461761357757No Hit
GCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2960.6126461761357757No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2940.608506674945669No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2910.6022974231605092No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2880.5960881713753492No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2880.5960881713753492No Hit
GCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2870.594018420780296No Hit
TCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2870.594018420780296No Hit
ACCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2850.5898789195901893No Hit
GCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2730.5650419124495498No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2700.55883266066439No Hit
CCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2650.5484839076891235No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2640.5464141570940702No Hit
CCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2640.5464141570940702No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2590.5360654041188037No Hit
ACCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2580.5339956535237503No Hit
ACCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2510.5195073993583773No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2500.517437648763324No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2500.517437648763324No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2440.5050191451930043No Hit
CCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2440.5050191451930043No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.4988098934078444No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2380.49260064162268447No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2360.48846114043257793No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2290.4739728862672048No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2280.47190313567215153No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2230.461554382696885No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2230.461554382696885No Hit
CCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2220.45948463210183177No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2220.45948463210183177No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2210.45741488150677845No Hit
GCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2170.4491358791265653No Hit
ACCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2170.4491358791265653No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2150.44499637793645863No Hit
GCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2120.4387871261512987No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2100.43464762496119214No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2070.4284383731760323No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2070.4284383731760323No Hit
ACCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1950.40360136603539276No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1940.40153161544033944No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1930.3994618648452862No Hit
CCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1890.39118286246507294No Hit
CCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1880.3891131118700197No Hit
ACCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1880.3891131118700197No Hit
CCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1830.3787643588947532No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.3684156059194867No Hit
TCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1760.3642761047293801No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1740.3601366035392735No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.34771809996895375No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.33322984580358067No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1590.3290903446134741No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1590.3290903446134741No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.3228810928283142No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1550.3208113422332609No Hit
GCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1550.3208113422332609No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1520.314602090448101No Hit
CCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1510.3125323398530477No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA1500.3104625892579944No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1480.3063230880678878No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.3063230880678878No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.3042533374728345No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1440.2980440856876746No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.27734657973714166No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1310.2711373279519818No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1300.2690675773569285No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1270.2628583255717686No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1240.25664907378660873No Hit
ACCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1220.25250957259650214No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1210.2504398220014488No Hit
CCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1170.24216081962123562No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1160.24009106902618235No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1150.23802131843112906No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1140.23595156783607577No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1130.23388181724102244No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1120.23181206664596915No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1120.23181206664596915No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1110.22974231605091588No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1080.22353306426575598No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGA1070.22146331367070268No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1020.2111145606954362No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1020.2111145606954362No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1020.2111145606954362No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA990.20490530891027633No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA980.20283555831522299No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA970.20076580772016972No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA960.19869605712511643No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA950.19662630653006313No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA940.19455655593500984No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA930.19248680533995652No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA890.18420780295974334No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA890.18420780295974334No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.17799855117458346No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.17385904998447688No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA830.17178929938942358No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA780.1614405464141571No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA770.1593707958191038No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA740.1531615440339439No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA740.1531615440339439No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.15109179343889062No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA720.1490220428438373No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA710.14695229224878403No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.14695229224878403No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA700.1448825416537307No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.14281279105867745No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA690.14281279105867745No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA690.14281279105867745No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA670.13867328986857083No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA660.13660353927351754No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA650.13453378867846424No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.13453378867846424No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA640.13246403808341095No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA640.13246403808341095No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA630.13039428748835766No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA610.12625478629825107No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA600.12418503570319776No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA570.11797578391803788No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA570.11797578391803788No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.11590603332298458No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.10969678153782468No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA520.1076270309427714No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA520.1076270309427714No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA500.1034875297526648No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.10141777915761149No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.10141777915761149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)