FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50520
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA27565.45526524148852No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA10662.110055423594616No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7611.5063341250989708No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7341.4528899445764054No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7191.4231987331749802No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7181.4212193190815519No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6751.336104513064133No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6231.2331749802058591No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6051.1975455265241488No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5911.169833729216152No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5821.152019002375297No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5671.1223277909738716No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5301.049089469517023No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5211.031274742676168No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5181.0253365003958828No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5171.0233570863024546No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5101.009501187648456No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4960.9817893903404592No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4810.952098178939034No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4740.9382422802850356No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4680.9263657957244655No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4500.8907363420427553No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4490.888756927949327No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4450.8808392715756137No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4380.8669833729216153No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4070.8056215360253366No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4050.8016627078384799No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3950.7818685669041963No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3880.7680126682501979No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3800.7521773555027712No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3650.722486144101346No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3480.6888361045130641No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3450.6828978622327792No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3260.6452889944576405No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3210.6353919239904988No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3190.631433095803642No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3100.613618368962787No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2920.5779889152810769No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2900.5740300870942201No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2850.5641330166270784No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2720.53840063341251No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA2690.5324623911322248No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2210.4374505146476643No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2140.4235946159936659No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2090.41369754552652416No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1970.38994457640538405No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1740.34441805225653205No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGA1670.33056215360253366No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1510.2988915281076801No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1510.2988915281076801No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA1360.269200316706255No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA1200.23752969121140144No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1180.23357086302454474No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA1130.22367379255740302No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1110.21971496437054633No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA970.19200316706254947No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA890.17616785431512272No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA860.1702296120348377No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.1702296120348377No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.166270783847981No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA830.16429136975455266No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA820.1623119556611243No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA790.15637371338083927No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA780.15439429928741094No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA770.15241488519398258No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA760.15043547110055422No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA740.14647664291369755No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.14449722882026922No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA720.14251781472684086No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA710.1405384006334125No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGA700.13855898653998416No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA670.1326207442596991No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA670.1326207442596991No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA650.12866191607284244No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA650.12866191607284244No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA640.1266825019794141No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA640.1266825019794141No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA640.1266825019794141No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGA640.1266825019794141No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA630.12470308788598573No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA610.12074425969912905No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA610.12074425969912905No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.11678543151227237No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA580.11480601741884401No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA570.11282660332541568No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA570.11282660332541568No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA560.11084718923198733No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA540.10688836104513064No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA540.10688836104513064No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA540.10688836104513064No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA530.10490894695170229No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA530.10490894695170229No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA530.10490894695170229No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGA530.10490894695170229No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA520.10292953285827396No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA520.10292953285827396No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA510.10095011876484561No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA510.10095011876484561No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.10095011876484561No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA510.10095011876484561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)