FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50554
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA21494.25090002769316No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8611.7031293270562173No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA6501.2857538473711279No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5851.1571784626340151No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5841.1552003797919057No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA5791.1453099655813586No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5631.1136606401076077No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5541.0958578945286228No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5271.0424496577916684No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4540.8980496103176802No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4520.8940934446334613No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4470.884203030422914No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4460.8822249475808046No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4060.8031016338964275No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4040.7991454682122087No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4030.7971673853700992No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4010.7932112196858805No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3730.7378249001068165No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3690.7299125687383788No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3560.7041974917909563No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3560.7041974917909563No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3530.6982632432646279No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3520.6962851604225184No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3520.6962851604225184No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3430.6784824148435337No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3360.6646358349487677No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3330.6587015864224394No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3130.6191399295802509No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3130.6191399295802509No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3080.6092495153697036No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2830.559797444316968No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2700.5340823673695454No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2680.5301262016853265No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2660.5261700360011078No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2600.5143015389484511No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2560.5063892075800135No Hit
TCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2530.5004549590536852No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2500.49452071052735685No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2430.4806741306325909No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2350.46484946789571546No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2320.4589152193693872No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2210.43715630810618344No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2160.42726589389563635No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2130.42133164536930806No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1960.3877042370534478No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1820.3600110772639158No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.35012066305336864No Hit
ACCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1700.3362740831586027No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1670.3303398346322744No Hit
GCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1660.32836175179016497No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA1650.32638366894805554No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1600.3164932547375084No Hit
TCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1600.3164932547375084No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1590.31451517189539896No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1560.3085809233690707No Hit
TCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1520.300668592000633No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1500.29671242631641415No Hit
TCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1500.29671242631641415No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1490.2947343434743047No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.2927562606321953No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1460.2888000949479764No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1440.28484392926375757No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1410.2789096807374293No Hit
GCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1320.26110693515844446No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1300.25715076947422555No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1300.25715076947422555No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1300.25715076947422555No Hit
TCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1290.2551726866321161No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1280.25319460379000674No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1250.24726035526367843No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA1230.2433041895794596No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1200.23736994105313128No Hit
ACCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1180.23341377536891245No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1170.23143569252680302No Hit
CCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1160.2294576096846936No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1150.22747952684258416No Hit
ACCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1120.22154527831625587No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1120.22154527831625587No Hit
TCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1120.22154527831625587No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1110.21956719547414644No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA1100.21758911263203706No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1100.21758911263203706No Hit
TCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1090.2156110297899276No Hit
GCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1060.20967678126359932No Hit
GCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1050.2076986984214899No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1020.2017644498951616No Hit
TCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1020.2017644498951616No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA1020.2017644498951616No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1010.19978636705305217No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1010.19978636705305217No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA1000.19780828421094276No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA990.19583020136883333No Hit
GCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA980.1938521185267239No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA980.1938521185267239No Hit
ACCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA960.18989595284250504No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA940.18593978715828618No Hit
TCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA940.18593978715828618No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA920.18198362147406735No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA920.18198362147406735No Hit
CCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA910.1800055386319579No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA900.1780274557898485No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA890.17604937294773906No Hit
CCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA890.17604937294773906No Hit
GCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA880.1740712901056296No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA880.1740712901056296No Hit
GCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA870.1720932072635202No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.17011512442141077No Hit
GCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA860.17011512442141077No Hit
ACCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA850.16813704157930134No Hit
CCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA840.16615895873719191No Hit
TCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA830.16418087589508248No Hit
ACCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA830.16418087589508248No Hit
ACCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA810.16022471021086362No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA810.16022471021086362No Hit
CCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA810.16022471021086362No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA800.1582466273687542No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA770.1523123788424259No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.1503342960003165No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.1503342960003165No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.1503342960003165No Hit
GCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA750.14835621315820707No Hit
ACCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA750.14835621315820707No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA740.14637813031609764No Hit
ACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA730.1444000474739882No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA730.1444000474739882No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA730.1444000474739882No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA720.14242196463187878No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGA720.14242196463187878No Hit
CCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA720.14242196463187878No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.14044388178976935No Hit
CCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA680.13450963326344106No Hit
CCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA670.13253155042133163No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA660.13055346757922223No Hit
TCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA650.12857538473711277No Hit
CCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA650.12857538473711277No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.12659730189500337No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA630.12461921905289393No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.12461921905289393No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.12066305336867508No Hit
ACCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA580.1147288048423468No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.11275072200023738No Hit
CCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA550.10879455631601853No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA550.10879455631601853No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA540.10681647347390909No Hit
CCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA530.10483839063179966No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA520.10286030778969024No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.10286030778969024No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA520.10286030778969024No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.1008822249475808No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA510.1008822249475808No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.1008822249475808No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.1008822249475808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)