FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35859
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA13093.650408544577372No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA10482.922557795811372No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4951.3804065924872417No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA3991.112691374550322No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3661.020664268384506No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3530.984411165955548No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3510.9788337655818623No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3330.9286371622186899No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3200.8923840597897321No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3080.858919657547617No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3060.8533422571739313No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2990.8338213558660308No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2920.8143004545581305No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2910.8115117543712875No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2860.7975682534370729No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2860.7975682534370729No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2830.7892021528765442No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2790.7780473521291726No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2710.7557377506344293No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2680.7473716500739006No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2670.7445829498870576No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2630.733428149139686No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2550.7111185476449426No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2550.7111185476449426No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2530.7055411472712569No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2430.6776541454028278No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.6720767450291419No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2300.6414010429738699No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA2260.6302462422264983No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2220.6190914414791266No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2210.6163027412922837No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2180.6079366407317549No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2160.6023592403580691No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2160.6023592403580691No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2130.5939931397975403No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2090.5828383390501687No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2080.5800496388633258No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2060.57447223848964No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2060.57447223848964No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2050.5716835383027971No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2020.5633174377422683No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2020.5633174377422683No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2000.5577400373685825No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1990.5549513371817396No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1960.5465852366212108No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1940.541007836247525No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1910.5326417356869964No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1890.5270643353133104No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1840.513120834379096No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1840.513120834379096No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1800.5019660336317243No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.4963886332580384No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1760.49081123288435263No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1760.49081123288435263No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1730.48244513232382386No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.47407903176329513No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1680.4685016313896093No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1680.4685016313896093No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1660.46292423101592345No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1630.4545581304553947No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1620.4517694302685518No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1580.4406146295211802No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1570.4378259293343373No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1570.4378259293343373No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1550.4322485289606514No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1540.4294598287738085No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.42667112858696565No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.412727627652751No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1450.40436152709222234No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1420.39599542653169356No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1420.39599542653169356No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1410.3932067263448507No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1380.38484062578432193No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1370.382051925597479No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1350.3764745252237932No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1350.3764745252237932No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1320.3681084246632645No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1280.3569536239158928No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1280.3569536239158928No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1240.3457988231685211No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1240.3457988231685211No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1230.34301012298167827No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA1220.3402214227948353No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1190.3318553222343066No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1080.30117962017903455No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1080.30117962017903455No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1040.2900248194316629No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.28723611924482No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1010.28165871887113414No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA940.2621378175632338No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.253771717002705No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA900.25098301681586216No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.2398282160684905No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA840.23425081569480466No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA820.22867341532111884No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA780.2175186145737472No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.21194121420006137No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA740.2063638138263755No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA730.20357511363953262No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA720.2007864134526897No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.1784768119579464No Hit
TCCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA630.17568811177110347No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA630.17568811177110347No Hit
ACCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA620.17289941158426056No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.17289941158426056No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.17289941158426056No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.17289941158426056No Hit
GCCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA600.16732201121057474No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.16732201121057474No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.16732201121057474No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.16174461083688893No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA570.158955910650046No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.1561672104632031No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA560.1561672104632031No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA550.15337851027636018No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA550.15337851027636018No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.14780110990267437No Hit
TCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA520.14501240971583146No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA520.14501240971583146No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.14222370952898855No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA500.13943500934214562No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.13943500934214562No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.1338576089684598No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.1338576089684598No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.1310689087816169No Hit
TCCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA470.1310689087816169No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.1310689087816169No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA460.128280208594774No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.12549150840793108No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.12549150840793108No Hit
ACCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA440.12270280822108816No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA440.12270280822108816No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.12270280822108816No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.12270280822108816No Hit
GCCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA440.12270280822108816No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA440.12270280822108816No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.12270280822108816No Hit
GCCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA430.11991410803424525No Hit
CCCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA430.11991410803424525No Hit
ACCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA410.11433670766055942No Hit
ACCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA410.11433670766055942No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.11154800747371652No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA400.11154800747371652No Hit
TCCTACGGGGTGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA400.11154800747371652No Hit
ACCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA390.1087593072868736No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.1087593072868736No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA390.1087593072868736No Hit
ACCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA390.1087593072868736No Hit
TCCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA380.10597060710003069No Hit
GCCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA380.10597060710003069No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.10597060710003069No Hit
TCCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA380.10597060710003069No Hit
CCCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA380.10597060710003069No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA370.10318190691318775No Hit
GCCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA370.10318190691318775No Hit
GCCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA370.10318190691318775No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA360.10039320672634484No Hit
GCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA360.10039320672634484No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA360.10039320672634484No Hit
ACCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA360.10039320672634484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)