FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13662
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5944.3478260869565215No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2441.7859756990191773No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1721.2589664763577808No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1581.156492460840287No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1501.0979358805445762No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1451.061338017859757No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1441.0540184453227932No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1411.0320597277119017No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1391.0174205826379739No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.9076269945835164No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.9076269945835164No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1110.8124725516029864No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1090.7978334065290588No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.7758746889181671No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.7758746889181671No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.7539159713072756No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1030.7539159713072756No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.739276826233348No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1000.7319572536963841No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.7026789635485288No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA950.695359391011565No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.6807202459376372No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.6734006734006733No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.6734006734006733No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.6514419557897819No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.6514419557897819No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.6148440931049627No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA820.6002049480310351No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA800.5855658029571073No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA790.5782462304201434No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.5636070853462157No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.5343287951983604No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.5196896501244328No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.5123700775874689No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA600.4391743522178305No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.43185477968086666No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.4245352071439028No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.4245352071439028No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.4245352071439028No Hit
GCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA550.40257648953301123No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.40257648953301123No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA540.3952569169960474No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA540.3952569169960474No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.38793734445908357No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.3732981993851559No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.3732981993851559No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.3732981993851559No Hit
TCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA500.36597862684819205No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA500.36597862684819205No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.36597862684819205No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.35865905431122824No Hit
TCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA490.35865905431122824No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.3440199092373005No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.33670033670033667No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA440.322061191626409No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.3147416190894452No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA430.3147416190894452No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.30742204655248134No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA410.30010247401551754No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA400.2927829014785536No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA380.27814375640462596No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA380.27814375640462596No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.27082418386766216No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA370.27082418386766216No Hit
TCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA360.2635046113306983No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA360.2635046113306983No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA350.25618503879373444No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA350.25618503879373444No Hit
ACCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA340.24886546625677058No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA340.24886546625677058No Hit
CCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA330.24154589371980675No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.24154589371980675No Hit
TCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA320.23422632118284292No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA310.2269067486458791No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.21958717610891526No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.21958717610891526No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA290.2122676035719514No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA290.2122676035719514No Hit
GCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA290.2122676035719514No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA290.2122676035719514No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA290.2122676035719514No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA280.20494803103498754No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA280.20494803103498754No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA270.1976284584980237No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA270.1976284584980237No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA260.19030888596105988No Hit
GCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA250.18298931342409602No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA250.18298931342409602No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA250.18298931342409602No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA240.1756697408871322No Hit
TCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA240.1756697408871322No Hit
GCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA240.1756697408871322No Hit
ACCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA240.1756697408871322No Hit
GCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA240.1756697408871322No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA230.16835016835016833No Hit
ACCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA230.16835016835016833No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA220.1610305958132045No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA220.1610305958132045No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA220.1610305958132045No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA220.1610305958132045No Hit
ACCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA210.15371102327624067No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA210.15371102327624067No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA200.1463914507392768No Hit
TCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA200.1463914507392768No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA200.1463914507392768No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA200.1463914507392768No Hit
ACCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA200.1463914507392768No Hit
TCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA200.1463914507392768No Hit
TCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA200.1463914507392768No Hit
CCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA200.1463914507392768No Hit
GCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA190.13907187820231298No Hit
ACCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA190.13907187820231298No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA190.13907187820231298No Hit
CCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA190.13907187820231298No Hit
ACCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA190.13907187820231298No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA190.13907187820231298No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA180.13175230566534915No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA180.13175230566534915No Hit
CCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA180.13175230566534915No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA180.13175230566534915No Hit
ACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA170.12443273312838529No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA170.12443273312838529No Hit
ACCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA170.12443273312838529No Hit
CCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA170.12443273312838529No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA170.12443273312838529No Hit
TCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA160.11711316059142146No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA160.11711316059142146No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA160.11711316059142146No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA160.11711316059142146No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA160.11711316059142146No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA160.11711316059142146No Hit
ACCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA150.10979358805445763No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA150.10979358805445763No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGGGAAACCCTGA150.10979358805445763No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA140.10247401551749377No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA140.10247401551749377No Hit
GCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA140.10247401551749377No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA140.10247401551749377No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGA140.10247401551749377No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA140.10247401551749377No Hit
TCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA140.10247401551749377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)