FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19616
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7623.884584013050571No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA5022.559135399673736No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2761.4070146818923328No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1950.9940864600326265No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1940.9889885807504079No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1890.9634991843393148No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.922716150081566No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1760.897226753670473No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1660.8462479608482872No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1660.8462479608482872No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.7544861337683524No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.7544861337683524No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1470.7493882544861338No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1440.734094616639478No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1390.708605220228385No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1370.6984094616639478No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.6933115823817292No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.6423327895595432No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1260.6423327895595432No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1260.6423327895595432No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1260.6423327895595432No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.6423327895595432No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1250.6372349102773247No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1220.6219412724306688No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.6168433931484503No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1180.6015497553017944No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1170.5964518760195758No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1150.5862561174551387No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1150.5862561174551387No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1140.58115823817292No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1110.5658646003262643No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1110.5658646003262643No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1090.5556688417618271No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1070.54547308319739No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1040.5301794453507341No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.5250815660685155No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1020.5199836867862969No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1000.5097879282218597No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1000.5097879282218597No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA990.5046900489396411No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA980.4995921696574225No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA980.4995921696574225No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA970.4944942903752039No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA960.48939641109298526No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.48939641109298526No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA950.4842985318107667No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.47920065252854815No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.4741027732463295No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA920.46900489396411094No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.45371125611745516No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA880.4486133768352365No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA860.4384176182707994No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA850.4333197389885807No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA850.4333197389885807No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.4282218597063622No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.4282218597063622No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA820.41802610114192496No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA810.4129282218597064No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA800.40783034257748774No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA790.4027324632952692No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA790.4027324632952692No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA780.39763458401305063No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA770.39253670473083196No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA760.3874388254486134No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA760.3874388254486134No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.3874388254486134No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA750.3823409461663948No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.3721451876019576No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.3721451876019576No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA720.367047308319739No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.36194942903752036No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA710.36194942903752036No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA700.3568515497553018No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.3568515497553018No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA680.3466557911908646No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA640.3262642740619902No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.316068515497553No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.316068515497553No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA610.3109706362153344No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA600.30587275693311583No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.2956769983686786No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA570.29057911908646No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA560.28548123980424145No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA560.28548123980424145No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA560.28548123980424145No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA550.28038336052202284No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.2701876019575856No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.25999184339314846No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.25999184339314846No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.24979608482871124No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.23450244698205547No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.23450244698205547No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA440.22430668841761825No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA420.2141109298531811No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA420.2141109298531811No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA400.20391517128874387No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA400.20391517128874387No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA370.1886215334420881No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA340.1733278955954323No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.1733278955954323No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.1682300163132137No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA290.1478384991843393No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA290.1478384991843393No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA280.14274061990212072No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA280.14274061990212072No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA270.13764274061990212No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA270.13764274061990212No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGA270.13764274061990212No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA260.13254486133768353No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA260.13254486133768353No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA260.13254486133768353No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA260.13254486133768353No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA250.12744698205546492No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA240.12234910277324632No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.12234910277324632No Hit
TCCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA240.12234910277324632No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.12234910277324632No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA240.12234910277324632No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA230.11725122349102773No Hit
TCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA230.11725122349102773No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA230.11725122349102773No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA220.11215334420880912No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA220.11215334420880912No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA220.11215334420880912No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA220.11215334420880912No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA220.11215334420880912No Hit
TCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA210.10705546492659054No Hit
ACCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA210.10705546492659054No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA210.10705546492659054No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA210.10705546492659054No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA200.10195758564437193No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA200.10195758564437193No Hit
GCCTACGGGAGGCTGTAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA200.10195758564437193No Hit
ACCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA200.10195758564437193No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA200.10195758564437193No Hit
ACCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA200.10195758564437193No Hit
GCCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA200.10195758564437193No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA200.10195758564437193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)