FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48541
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA17963.6999649780597847No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA11292.325868853134464No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6521.3431944129704787No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA5481.1289425434169054No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4730.9744339836426937No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4630.9538328423394656No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4540.9352918151665602No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4320.8899693042994582No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4190.8631878206052614No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA3930.8096248532168683No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3900.8034445108258996No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3880.799324282565254No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3740.7704826847407347No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3400.7004388043097587No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3280.675717434745885No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3220.663356749963948No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3080.6345151521394286No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2960.6097937825755547No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2910.5994932119239407No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2760.5685914999690983No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2750.5665313858387755No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2740.5644712717084527No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2720.560351043447807No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2710.5582909293174841No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2650.5459302445355473No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2650.5459302445355473No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2650.5459302445355473No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2630.5418100162749016No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2620.5397499021445787No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2620.5397499021445787No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2600.5356296738839331No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2590.5335695597536103No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2530.5212088749716735No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2530.5212088749716735No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2510.5170886467110278No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2420.49854761953812243No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA2410.4964875054077996No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2340.48206670649553984No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.48000659236521703No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2310.4758863641045714No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2290.47176613584392574No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2140.4408644238890835No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2120.4367441956284378No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2110.434684081498115No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2090.4305638532374694No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2070.42644362497682375No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1980.40790259780391835No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1970.40584248367359554No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1960.40378236954327273No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1930.3976020271523042No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1910.3934817988916586No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1910.3934817988916586No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1900.39142168476133576No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1870.38524134237036733No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1860.3831812282400445No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1860.3831812282400445No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1850.3811211141097217No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1830.37700088584907604No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.3646402010671391No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1750.36051997280649345No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1670.3440390597639109No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1660.3419789456335881No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1650.3399188315032653No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1630.3357986032426197No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1580.3254980325910055No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1580.3254980325910055No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1570.3234379184606827No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1540.3172575760697143No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.3151974619393914No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1520.3131373478090686No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1510.3110772336787458No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGA1510.3110772336787458No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1510.3110772336787458No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1410.29047609237551764No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1410.29047609237551764No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1400.28841597824519477No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1400.28841597824519477No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1380.28429574998454915No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1370.28223563585422634No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1360.2801755217239035No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1320.2719350652026122No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1300.26781483694196656No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1290.26575472281164375No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1280.26369460868132094No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1250.2575142662903525No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1240.2554541521600297No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA1240.2554541521600297No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1230.25339403802970684No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1220.251333923899384No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1220.251333923899384No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1210.24927380976906122No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.24309346737809273No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1170.24103335324776992No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1160.2389732391174471No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1160.2389732391174471No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1130.23279289672647865No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1120.23073278259615584No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1120.23073278259615584No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1090.22455244020518736No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1060.2183720978142189No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1020.21013164129292766No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1000.206011413032282No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA970.1998310706413135No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.18747038585937661No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA900.1854102717290538No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.1771698152077625No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA860.1771698152077625No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA850.17510970107743967No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.17304958694711686No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA830.17098947281679405No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA810.1668692445561484No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA800.1648091304258256No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA800.1648091304258256No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA800.1648091304258256No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA780.16068890216517995No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA770.15862878803485714No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.1565686739045343No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.1565686739045343No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA760.1565686739045343No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA750.1545085597742115No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA740.15244844564388868No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.15038833151356587No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.15038833151356587No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.15038833151356587No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA700.14420798912259739No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA690.14214787499227458No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA660.1359675326013061No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA650.13390741847098328No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.13390741847098328No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.13390741847098328No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA650.13390741847098328No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA650.13390741847098328No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA640.13184730434066047No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA630.12978719021033766No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.12154673368904637No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.11948661955872356No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA570.11742650542840073No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA570.11742650542840073No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA570.11742650542840073No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.11536639129807792No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA550.11330627716775508No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA540.11124616303743227No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA540.11124616303743227No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA540.11124616303743227No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA530.10918604890710945No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA520.10712593477678664No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA520.10712593477678664No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.10506582064646383No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.103005706516141No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGA490.10094559238581818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)