FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19807
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA10645.371838239006412No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3921.9790982985813097No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3511.772100772454183No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2931.47927500378654No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2811.4186903619932347No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2671.348008279901045No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2631.3278133993032766No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2591.3076185187055083No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2541.2823749179582975No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2341.1814005149694553No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2291.1561569142222445No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2231.125864593325592No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2131.0753773918311706No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2081.0501337910839603No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1950.9845004291412126No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1950.9845004291412126No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1920.9693542686928864No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1890.9542081082445599No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1800.908769626899581No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1790.9037209067501389No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.8986721866006967No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.8936234664512547No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.8835260261523703No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.8481849851062756No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.8431362649568335No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1640.8279901045085071No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1620.8178926642096228No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1600.8077952239107387No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1520.7674054627152016No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.7573080224163175No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.7472105821174332No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.737113141818549No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1320.6664310597263593No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.6361387388297067No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1200.6058464179330539No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1190.6007976977836118No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1170.5907002574847277No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1140.5755540970364013No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.5553592164386328No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1080.5452617761397486No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1080.5452617761397486No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5402130559903064No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5402130559903064No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5402130559903064No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA940.47457969404755895No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA870.43923865300146414No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA850.42914121270257993No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA850.42914121270257993No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA830.4190437724036956No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA810.4089463321048114No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA800.40389761195536933No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.388751451507043No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA740.3736052910587166No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.3685565709092745No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA720.3635078507598324No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA690.34836169031150604No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA680.3433129701620639No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA660.33321552986317965No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA650.3281668097137376No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.3281668097137376No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA640.32311808956429544No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA640.32311808956429544No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA620.31302064926541123No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA580.29282576866764276No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA580.29282576866764276No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA570.28777704851820063No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA560.2827283283687585No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA550.2776796082193164No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA540.2726308880698743No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA520.2625334477709901No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA520.2625334477709901No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA510.25748472762154795No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA500.2524360074721058No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.2524360074721058No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA500.2524360074721058No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA500.2524360074721058No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.2473872873226637No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.2473872873226637No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA480.2423385671732216No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA480.2423385671732216No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA470.23728984702377948No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA470.23728984702377948No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA470.23728984702377948No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA460.23224112687433734No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA450.22719240672489524No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA450.22719240672489524No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA450.22719240672489524No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA450.22719240672489524No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.22719240672489524No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA450.22719240672489524No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.22214368657545314No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA440.22214368657545314No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.22214368657545314No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA430.217094966426011No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA430.217094966426011No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA430.217094966426011No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA430.217094966426011No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA420.2120462462765689No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA390.19690008582824253No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA390.19690008582824253No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA380.1918513656788004No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA380.1918513656788004No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA380.1918513656788004No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA360.1817539253799162No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA360.1817539253799162No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA350.17670520523047406No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA350.17670520523047406No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA350.17670520523047406No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA340.17165648508103196No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA340.17165648508103196No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA340.17165648508103196No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA340.17165648508103196No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA340.17165648508103196No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.16660776493158982No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.16660776493158982No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.16660776493158982No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA320.16155904478214772No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA310.15651032463270562No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA310.15651032463270562No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA310.15651032463270562No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA300.15146160448326348No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA300.15146160448326348No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA300.15146160448326348No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA300.15146160448326348No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA290.14641288433382138No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA280.14136416418437925No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA280.14136416418437925No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA270.13631544403493714No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA270.13631544403493714No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA270.13631544403493714No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.13631544403493714No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.13631544403493714No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA260.13126672388549504No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA260.13126672388549504No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA260.13126672388549504No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA260.13126672388549504No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA260.13126672388549504No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA240.1211692835866108No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA240.1211692835866108No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA230.11612056343716867No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA230.11612056343716867No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA230.11612056343716867No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA230.11612056343716867No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA230.11612056343716867No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA230.11612056343716867No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA220.11107184328772657No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA220.11107184328772657No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA220.11107184328772657No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA220.11107184328772657No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA220.11107184328772657No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA220.11107184328772657No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA210.10602312313828445No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA210.10602312313828445No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA210.10602312313828445No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA210.10602312313828445No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGGGAAACCCTGA210.10602312313828445No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA200.10097440298884233No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA200.10097440298884233No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA200.10097440298884233No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA200.10097440298884233No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA200.10097440298884233No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA200.10097440298884233No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA200.10097440298884233No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA200.10097440298884233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)