FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36229
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA12953.574484528968506No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4991.3773496370311076No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3590.9909188771426205No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3420.9439951420133044No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3390.9357144828728367No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3140.8667089900356069No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2900.8004637169118662No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2880.7949432774848879No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2620.7231775649341687No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2520.6955753677992769No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2500.6900549283722984No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2380.6569322918104281No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2380.6569322918104281No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2370.654172072096939No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2340.6458914129564713No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2290.6320903143890254No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2200.6072483369676226No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2130.5879267989731982No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2110.5824063595462199No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2090.5768859201192414No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1960.541003063843882No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1950.5382428441303928No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1930.5327224047034144No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1920.5299621849899252No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1910.527201965276436No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1900.5244417455629469No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA1890.5216815258494576No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1890.5216815258494576No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1860.5134008667089901No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1850.5106406469955008No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1820.5023599878550332No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1800.49683954842805483No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.4885588892875873No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.4692373512931629No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1630.44991581329873853No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.43059427530431416No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.4057522978829115No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA1400.38643075988848713No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1390.3836705401749979No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.3753898810345303No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.369869441607552No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1310.36158878246708437No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1300.35882856275359515No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1280.3533081233266168No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.34778768389963843No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.3036241684838113No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1090.30086394877032213No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.2953435093433437No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1070.2953435093433437No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA990.2732617516354302No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA960.2649810924949626No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA870.24013911507355987No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA810.22357779679262468No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA800.2208175770791355No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA800.2208175770791355No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGA790.2180573573656463No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA780.21529713765215708No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA770.21253691793866794No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA750.20701647851168953No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA750.20701647851168953No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA720.19873581937122198No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGA700.19321537994424356No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGA680.18769494051726515No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA680.18769494051726515No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA650.17941428137679757No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA650.17941428137679757No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.1766540616633084No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA640.1766540616633084No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA630.17389384194981922No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA620.17113362223633No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA620.17113362223633No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA620.17113362223633No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA610.16837340252284083No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.1656131828093516No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA600.1656131828093516No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA580.16009274338237323No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA570.15733252366888403No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA560.15457230395539487No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA560.15457230395539487No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.14905186452841646No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA530.1462916448149273No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA510.14077120538794888No Hit
ACCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA500.13801098567445969No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.1352507659609705No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.1352507659609705No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA480.1324905462474813No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA480.1324905462474813No Hit
GCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA480.1324905462474813No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA470.1297303265339921No Hit
TCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA460.12697010682050291No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA460.12697010682050291No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.12697010682050291No Hit
TCCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA450.12420988710701371No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA450.12420988710701371No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.12420988710701371No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.12420988710701371No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA450.12420988710701371No Hit
GCCTACGGGAGGCTGCAGCCAGGAATCTTGCGCAATGGGCGAAAGCCTGA450.12420988710701371No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA440.12144966739352453No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA440.12144966739352453No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA440.12144966739352453No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.11868944768003532No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA430.11868944768003532No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA430.11868944768003532No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA420.11592922796654613No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA420.11592922796654613No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.11316900825305695No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.11316900825305695No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA410.11316900825305695No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA410.11316900825305695No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.11316900825305695No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA410.11316900825305695No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA410.11316900825305695No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA400.11040878853956775No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA400.11040878853956775No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.11040878853956775No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.11040878853956775No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA400.11040878853956775No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA400.11040878853956775No Hit
TCCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA400.11040878853956775No Hit
TCCTATGGGGCGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGA390.10764856882607854No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA390.10764856882607854No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA390.10764856882607854No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA380.10488834911258936No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA380.10488834911258936No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA380.10488834911258936No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA380.10488834911258936No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.10488834911258936No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.10488834911258936No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.10212812939910017No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA370.10212812939910017No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA370.10212812939910017No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA370.10212812939910017No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA370.10212812939910017No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.10212812939910017No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA370.10212812939910017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)