FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18370
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8934.861186717474142No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2921.5895481763745236No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2301.2520413718018508No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2191.1921611322808927No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2001.0887316276537833No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1961.0669569951007076No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1800.979858464888405No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1760.9580838323353293No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1660.9036472509526402No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.8764289602612956No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1590.8655416439847577No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1590.8655416439847577No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.8165487207403375No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.8002177463255309No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.7784431137724551No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1420.7729994556341862No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1390.7566684812193795No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.7403375068045727No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.7294501905280348No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.7294501905280348No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1320.718562874251497No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1300.7076755579749592No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1220.6641262928688078No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.6586826347305389No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1170.6369080021774632No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1170.6369080021774632No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1160.6314643440391944No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1130.6151333696243876No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1130.6151333696243876No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1110.6042460533478498No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5824714207947741No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.5498094719651606No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA970.528034839412085No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA970.528034839412085No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA950.5171475231355471No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.5117038649972782No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.5117038649972782No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA930.5062602068590092No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.4844855743059336No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.4844855743059336No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA880.47904191616766467No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.4518236254763201No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA820.4463799673380512No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA810.4409363091997823No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA770.41916167664670656No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.41916167664670656No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA750.40827436037016873No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.38105606967882416No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.3592814371257485No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.3538377789874796No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA610.3320631464344039No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.3157321720195972No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.28851388132825256No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.28307022318998365No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA520.28307022318998365No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA520.28307022318998365No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA510.27762656505171474No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.27762656505171474No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA440.23952095808383234No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.23407729994556342No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.23407729994556342No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA420.2286336418072945No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA420.2286336418072945No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA420.2286336418072945No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.2231899836690256No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA410.2231899836690256No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA410.2231899836690256No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA400.21774632553075668No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA400.21774632553075668No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA400.21774632553075668No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA390.21230266739248774No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA390.21230266739248774No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA390.21230266739248774No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA380.20685900925421882No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA360.195971692977681No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA350.19052803483941208No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.18508437670114317No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA340.18508437670114317No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA330.17964071856287425No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.17964071856287425No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA320.17419706042460534No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA320.17419706042460534No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA310.16875340228633642No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA310.16875340228633642No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA300.16330974414806748No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.16330974414806748No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.16330974414806748No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA300.16330974414806748No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA300.16330974414806748No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA290.1578660860097986No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA290.1578660860097986No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA290.1578660860097986No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA290.1578660860097986No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA290.1578660860097986No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA280.15242242787152968No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA280.15242242787152968No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA280.15242242787152968No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA280.15242242787152968No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA270.14697876973326074No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.14697876973326074No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA270.14697876973326074No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA270.14697876973326074No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA260.14153511159499182No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA260.14153511159499182No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA250.1360914534567229No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA250.1360914534567229No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA250.1360914534567229No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA250.1360914534567229No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA240.130647795318454No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA240.130647795318454No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA240.130647795318454No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA240.130647795318454No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.130647795318454No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA240.130647795318454No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA230.12520413718018508No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA230.12520413718018508No Hit
TCCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA230.12520413718018508No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA230.12520413718018508No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA230.12520413718018508No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA230.12520413718018508No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA230.12520413718018508No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA230.12520413718018508No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA220.11976047904191617No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA220.11976047904191617No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA220.11976047904191617No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA220.11976047904191617No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA220.11976047904191617No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA220.11976047904191617No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA220.11976047904191617No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA220.11976047904191617No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA220.11976047904191617No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA220.11976047904191617No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA210.11431682090364725No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA210.11431682090364725No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA210.11431682090364725No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA210.11431682090364725No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA210.11431682090364725No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA210.11431682090364725No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA200.10887316276537834No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA200.10887316276537834No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.10887316276537834No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA200.10887316276537834No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.10887316276537834No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA200.10887316276537834No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA190.10342950462710941No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA190.10342950462710941No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA190.10342950462710941No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA190.10342950462710941No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA190.10342950462710941No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA190.10342950462710941No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA190.10342950462710941No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA190.10342950462710941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)