FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18094
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7073.9073726097048747No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA3151.7409085884823698No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2991.652481485575329No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2701.4922073615563172No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2141.1827125013816735No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1861.0279650712943518No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.9837515198408312No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.967171438045761No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1730.9561180501823809No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1710.9450646623190009No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.8897977230021001No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.8290040897535095No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1440.7958439261633692No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1440.7958439261633692No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.7571570686415386No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.7516303747098486No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1330.7350502929147784No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1310.7239969050513982No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.6853100475295678No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1240.6853100475295678No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1230.6797833535978778No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1190.6576765778711174No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1170.6466231900077374No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1140.6300431082126672No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1130.6245164142809772No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5913562506908367No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5913562506908367No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1040.5747761688957665No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1020.5637227810323864No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.5581960871006963No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.5581960871006963No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1000.5526693931690063No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA990.5471426992373163No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA950.525035923510556No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.5084558417154857No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.5084558417154857No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA890.4918757599204156No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA890.4918757599204156No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA870.4808223720570355No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA860.47529567812534546No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.47529567812534546No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.4642422902619653No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA820.4531889023985851No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA800.44213551453520505No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA790.43660882060351497No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA780.43108212667182494No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.4255554327401348No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA760.42002873880844477No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA750.41450204487675474No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA750.41450204487675474No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA730.4034486570133746No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.4034486570133746No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA720.3979219630816846No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.39239526914999445No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.38134188128661434No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.3758151873549243No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.36476179949154414No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA660.36476179949154414No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.3592351055598541No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA630.348181717696474No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA620.3426550237647839No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.3426550237647839No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.3426550237647839No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA610.3371283298330939No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA610.3371283298330939No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.3371283298330939No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA590.3260749419697137No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.3260749419697137No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA580.3205482480380236No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.3150215541063336No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA570.3150215541063336No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA550.3039681662429535No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA540.2984414723112634No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA530.2929147783795733No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA530.2929147783795733No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA510.2818613905161932No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA510.2818613905161932No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA510.2818613905161932No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.2818613905161932No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA500.27633469658450316No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.27633469658450316No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.2708080026528131No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA480.26528130872112304No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.25975461478943296No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.25975461478943296No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.25975461478943296No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA460.2542279208577429No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.2542279208577429No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.2542279208577429No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA450.24870122692605282No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA450.24870122692605282No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA450.24870122692605282No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA450.24870122692605282No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA450.24870122692605282No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA440.24317453299436279No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA440.24317453299436279No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA440.24317453299436279No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.23764783906267273No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.23764783906267273No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA430.23764783906267273No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA420.23212114513098264No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA420.23212114513098264No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA420.23212114513098264No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA410.22659445119929256No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA410.22659445119929256No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.22106775726760253No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.22106775726760253No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.22106775726760253No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.22106775726760253No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA390.21554106333591247No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA390.21554106333591247No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA380.21001436940422238No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.20448767547253233No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.20448767547253233No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA370.20448767547253233No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA360.1989609815408423No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA350.19343428760915218No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA340.18790759367746215No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.18238089974577207No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.18238089974577207No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA320.176854205814082No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA320.176854205814082No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA320.176854205814082No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA310.17132751188239195No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA310.17132751188239195No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA300.1658008179507019No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA300.1658008179507019No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA280.15474743008732178No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA280.15474743008732178No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA270.1492207361556317No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.1492207361556317No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.1492207361556317No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA250.13816734829225158No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA250.13816734829225158No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA240.13264065436056152No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.13264065436056152No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.13264065436056152No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.13264065436056152No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA230.12711396042887144No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA220.12158726649718139No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA220.12158726649718139No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA210.11606057256549132No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA210.11606057256549132No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA210.11606057256549132No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA210.11606057256549132No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.11053387863380126No Hit
ACCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA200.11053387863380126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)