FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20233
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA12696.271931992289824No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4702.322937774922157No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3451.7051351752088173No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3331.6458261256363367No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3311.635941284040923No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3181.571689813670736No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3121.5420352888844957No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3051.5074383433005485No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA2891.4283596105372411No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2741.3542232985716405No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2531.2504324618197993No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2511.240547620224386No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2501.2356051994266792No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2471.220777937033559No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2411.1911234122473187No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2381.1762961498541986No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2381.1762961498541986No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2371.171353729056492No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2291.1318143626748383No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2201.0873325754954777No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2171.0725053131023576No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2171.0725053131023576No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2081.028023525922997No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2071.0230811051252904No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2020.9983690011367569No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2020.9983690011367569No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1980.9785993179459299No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1930.9538872139573964No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1930.9538872139573964No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1910.9440023723619829No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1880.9291751099688628No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1780.8797509019917956No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1680.8303266940147285No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1660.820441852419315No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1640.8105570108239016No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1610.7957297484307815No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1570.7759600652399545No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1450.7166510156674739No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1430.7067661740720604No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1420.7018237532743538No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1400.6919389116789404No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1270.627687441308753No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1260.6227450205110463No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1220.6029753373202195No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1170.5782632333316858No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1130.558493550140859No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA1110.5486087085454455No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1010.49918450056837843No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA920.45470271338901797No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA910.44976029259131123No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA890.4398754509958978No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA830.4102209262096575No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA810.4003360846142441No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA790.3904512430188306No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA770.3805664014234172No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA760.37562398062571045No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA730.3607967182325903No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA700.3459694558394702No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA700.3459694558394702No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.3459694558394702No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA690.34102703504176346No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA670.33114219344635004No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA670.33114219344635004No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.3261997726486433No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA660.3261997726486433No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.3261997726486433No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA650.32125735185093657No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.31137251025552315No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.30643008945781647No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.30148766866010973No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA600.296545247862403No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA590.2916028270646963No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.28171798546928284No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.28171798546928284No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.28171798546928284No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA570.28171798546928284No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA550.2718331438738694No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.2718331438738694No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA550.2718331438738694No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA520.25700588148074927No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA520.25700588148074927No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.25700588148074927No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA520.25700588148074927No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA490.24217861908762914No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.24217861908762914No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA480.23723619828992243No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.23723619828992243No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA480.23723619828992243No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.23723619828992243No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA480.23723619828992243No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA470.2322937774922157No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.2322937774922157No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA470.2322937774922157No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.2322937774922157No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA460.22735135669450898No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA460.22735135669450898No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.22735135669450898No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.22735135669450898No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.22240893589680227No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.22240893589680227No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA440.21746651509909556No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA430.21252409430138883No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.21252409430138883No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.20758167350368212No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA410.2026392527059754No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.2026392527059754No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA410.2026392527059754No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA400.1976968319082687No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.1976968319082687No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA400.1976968319082687No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA400.1976968319082687No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.1976968319082687No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA400.1976968319082687No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA400.1976968319082687No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA390.19275441111056196No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.19275441111056196No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.19275441111056196No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA380.18781199031285523No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA380.18781199031285523No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA380.18781199031285523No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.18781199031285523No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA370.18286956951514852No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA370.18286956951514852No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.18286956951514852No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.1779271487174418No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA360.1779271487174418No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA360.1779271487174418No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.1779271487174418No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.1729847279197351No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA350.1729847279197351No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA350.1729847279197351No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA340.16804230712202836No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.16309988632432165No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA330.16309988632432165No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA330.16309988632432165No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA330.16309988632432165No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA320.15815746552661494No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.15815746552661494No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA320.15815746552661494No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA320.15815746552661494No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA320.15815746552661494No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.15321504472890823No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA300.1482726239312015No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA300.1482726239312015No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA300.1482726239312015No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA300.1482726239312015No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA300.1482726239312015No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA290.1433302031334948No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA290.1433302031334948No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA290.1433302031334948No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA290.1433302031334948No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA290.1433302031334948No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA290.1433302031334948No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA290.1433302031334948No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.13838778233578808No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA280.13838778233578808No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.13838778233578808No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.13344536153808137No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.13344536153808137No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA260.12850294074037463No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA260.12850294074037463No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA260.12850294074037463No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA250.12356051994266792No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA240.11861809914496121No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA230.11367567834725449No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA230.11367567834725449No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA220.10873325754954778No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA220.10873325754954778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)