FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21149
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA11445.409239207527543No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6783.2058253345311836No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA4502.127760177786184No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3261.5414440399073244No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3081.456333632795877No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2991.4137784292401532No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2921.3806799375857013No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2731.2908411745236181No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2481.172632275757719No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2461.1631755638564472No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2311.0922502245969077No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2211.044966665090548No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2181.0307815972386403No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2171.0260532412880041No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2131.0071398174854604No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2110.9976831055841884No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2060.9740413258310086No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2060.9740413258310086No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2050.9693129698803726No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1990.9409428341765568No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1980.9362144782259209No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1900.8983876306208332No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1890.8936592746701971No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1870.8842025627689253No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.8558324270651094No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1790.8463757151638376No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1780.8416473592132016No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.8274622913612938No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.7943637997068419No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1670.7896354437562059No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1650.780178731854934No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1580.7470802402004822No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1570.7423518842498463No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1570.7423518842498463No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1560.7376235282992104No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1520.7187101044966665No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1490.7045250366447586No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1480.6997966806941227No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1470.6950683247434867No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1470.6950683247434867No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1450.6856116128422147No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1450.6856116128422147No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1450.6856116128422147No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1400.6619698330890349No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1390.657241477138399No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.6335996973852192No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1330.6288713414345831No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1320.6241429854839472No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1290.6099579176320393No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1250.5910444938294954No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.5863161378788595No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1240.5863161378788595No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.5721310700269516No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.5721310700269516No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1190.5626743581256797No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1140.5390325783724998No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1140.5390325783724998No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1140.5390325783724998No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1120.5295758664712279No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1110.524847510520592No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1080.5106624426686841No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5059340867180481No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1030.4870206629155043No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.4870206629155043No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.4870206629155043No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.4775639510142323No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.45392217126105255No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA950.4491938153104166No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA940.44446545935978066No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA900.4255520355572367No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA900.4255520355572367No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.4208236796066008No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.4208236796066008No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA880.4160953236559648No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA860.40663861175469285No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA850.401910255804057No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.39245354390278503No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.3688117641496052No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA760.35935505224833325No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.33098491654451745No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA690.32625656059388153No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA660.3120714927419736No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.3073431367913377No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.3073431367913377No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA600.2837013570381578No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.2789730010875219No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.260059577284978No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA490.23168944158116225No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA490.23168944158116225No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA400.18913423802543855No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA390.1844058820748026No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA370.17494917017353068No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA360.1702208142228947No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGA350.16549245827225872No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA350.16549245827225872No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA350.16549245827225872No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA340.16076410232162278No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA340.16076410232162278No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA330.1560357463709868No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.15130739042035085No Hit
CCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA300.1418506785190789No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA290.13712232256844295No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA260.12293725471653506No Hit
CCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA260.12293725471653506No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA260.12293725471653506No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA250.1182088987658991No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA230.10875218686462716No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA230.10875218686462716No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA230.10875218686462716No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA220.1040238309139912No Hit
GCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA220.1040238309139912No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA220.1040238309139912No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA220.1040238309139912No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA220.1040238309139912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)