FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14463
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2111.4588951116642468No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2031.4035815529281614No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1841.2722118509299591No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1841.2722118509299591No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1811.2514692664039273No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1751.2099840973518634No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1651.1408421489317568No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1641.1339279540897462No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1551.0717002005116505No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1531.057871810827629No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1481.0233008366175758No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.9679872778814907No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1360.9403304985134481No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1290.8919311346193736No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.8227891861992671No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1180.8158749913572565No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.7882182119892139No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.7398188480951393No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.6845052893590542No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.6845052893590542No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.6706768996750329No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.6706768996750329No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.6499343151490009No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.6430201203069903No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.629191730622969No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.5807923667288944No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.5807923667288944No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.5807923667288944No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.5462213925188412No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.5323930028348199No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.5323930028348199No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA760.5254788079928092No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.5047362234667773No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.5047362234667773No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.49782202862476665No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.490907833782756No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.47707944409873476No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.47707944409873476No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.47707944409873476No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.47707944409873476No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.47016524925672404No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.47016524925672404No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.47016524925672404No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA660.45633685957270276No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA660.45633685957270276No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.44942266473069215No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA640.4425084698886815No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA640.4425084698886815No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.43559427504667086No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.43559427504667086No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.43559427504667086No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.42868008020466014No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.42868008020466014No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.4217658853626495No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.4217658853626495No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.4217658853626495No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.40793749567862825No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.4010233008366176No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA570.39410910599460697No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA570.39410910599460697No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA570.39410910599460697No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA570.39410910599460697No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.3871949111525963No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.3871949111525963No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA540.37336652146857496No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA540.37336652146857496No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA530.36645232662656435No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA530.36645232662656435No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.3595381317845537No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.3595381317845537No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.3595381317845537No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.3595381317845537No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.3595381317845537No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.3595381317845537No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.3457097421005324No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.3457097421005324No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.33879554725852173No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.33879554725852173No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA480.33188135241651107No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA480.33188135241651107No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA480.33188135241651107No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA470.32496715757450045No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA470.32496715757450045No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.3111387678904792No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.3111387678904792No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.3111387678904792No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.3042245730484685No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.3042245730484685No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.29731037820645784No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.2903961833644472No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA410.28348198852243656No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.2765677936804259No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.2765677936804259No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.2765677936804259No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA390.2696535988384153No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA390.2696535988384153No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA370.25582520915439394No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA370.25582520915439394No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA350.2419968194703727No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA350.2419968194703727No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA350.2419968194703727No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA340.23508262462836202No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA330.22816842978635138No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA320.22125423494434074No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA310.21434004010233007No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA310.21434004010233007No Hit
TGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA310.21434004010233007No Hit
CGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA300.20742584526031943No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA300.20742584526031943No Hit
TGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA290.2005116504183088No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA280.19359745557629815No Hit
CGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA280.19359745557629815No Hit
TGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA280.19359745557629815No Hit
TGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA260.17976906589227684No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA250.1728548710502662No Hit
AGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA250.1728548710502662No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA250.1728548710502662No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA240.16594067620825553No Hit
CGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA230.1590264813662449No Hit
TGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA230.1590264813662449No Hit
CGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA230.1590264813662449No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA230.1590264813662449No Hit
TGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA220.15211228652423425No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA220.15211228652423425No Hit
TGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA210.1451980916822236No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA210.1451980916822236No Hit
AGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA210.1451980916822236No Hit
AGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA200.13828389684021294No Hit
AGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA200.13828389684021294No Hit
CGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA200.13828389684021294No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA190.1313697019982023No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA190.1313697019982023No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA190.1313697019982023No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA180.12445550715619166No Hit
TGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA180.12445550715619166No Hit
CGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA180.12445550715619166No Hit
AGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA180.12445550715619166No Hit
TGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA170.11754131231418101No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA170.11754131231418101No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA170.11754131231418101No Hit
AGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA170.11754131231418101No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA170.11754131231418101No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA170.11754131231418101No Hit
AGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA160.11062711747217037No Hit
TGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA160.11062711747217037No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA160.11062711747217037No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA160.11062711747217037No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA160.11062711747217037No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA160.11062711747217037No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA160.11062711747217037No Hit
AGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA160.11062711747217037No Hit
CGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA150.10371292263015972No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA150.10371292263015972No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA150.10371292263015972No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA150.10371292263015972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)