FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54055
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA27865.154009804828416No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA10872.010914808990843No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA7881.4577744889464435No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA7291.348626399038017No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA7181.328276755156785No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA7141.3208768846545185No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA7101.3134770141522523No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA6911.2783276292664878No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA6901.2764776616409212No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6761.2505781148829895No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA6591.2191286652483582No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA5871.0859309962075663No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA5741.0618814170752011No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA5531.0230320969383035No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA5521.021182129312737No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA5060.9360836185366755No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4970.9194339099065767No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4940.9138840070298769No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4930.9120340394043105No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4740.8768846545185459No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4700.8694847840162797No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4590.8491351401350477No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4540.8398853020072148No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4360.8065858847470169No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4200.776986402737952No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4140.7658865969845527No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4100.7584867264822865No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3990.7381370826010545No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3810.7048376653408566No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3680.6807880862084914No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3630.6715382480806587No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3500.6474886689482934No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3340.6178891869392286No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3230.5975395430579965No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3200.5919896401812969No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3190.5901396725557302No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3160.5845897696790306No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA3130.579039866802331No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2970.5494403847932661No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2890.5346406437887338No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2890.5346406437887338No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2740.5068911294052354No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2730.5050411617796688No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA2700.4994912589029692No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2660.492091388400703No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2150.39774303949680884No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2120.39219313662010913No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2100.388493201368976No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1960.3625936546110443No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1950.36074368698547776No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1840.3403940431042457No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1830.3385440754786791No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1770.32744426972527985No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1750.3237443344741467No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1710.3163444639718805No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1700.31449449634631393No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.31079456109518083No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1650.3052446582184812No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1640.30339469059291463No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1600.2959948200906484No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1570.2904449172139488No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1570.2904449172139488No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.28304504671168257No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1470.2719452409582832No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1470.2719452409582832No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.264545370456017No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1410.2608454352048839No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1410.2608454352048839No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1400.2589954675793174No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1390.2571454999537508No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1380.25529553232818425No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1350.2497456294514846No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1320.24419572657478494No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA1280.23679585607251874No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1270.2349458884469522No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1260.23309592082138564No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1250.2312459531958191No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1250.2312459531958191No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.22939598557025254No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1230.22754601794468596No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1200.2219961150679863No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1190.22014614744241975No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.21829617981685318No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1180.21829617981685318No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1180.21829617981685318No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1160.21459624456572013No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1160.21459624456572013No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1130.20904634168902045No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1110.20534640643788735No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1080.19979650356118767No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1080.19979650356118767No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1080.19979650356118767No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1070.19794653593562112No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.19609656831005456No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.19609656831005456No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.19609656831005456No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.19609656831005456No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1050.194246600684488No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.1868467301822218No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA1000.18499676255665526No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1000.18499676255665526No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA990.1831467949310887No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA980.18129682730552216No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA970.1794468596799556No Hit
TCCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA960.17759689205438906No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA950.17574692442882248No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.17389695680325595No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA900.16649708630098972No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.16464711867542317No Hit
TCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA880.16279715104985665No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA860.15909721579872352No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA850.15724724817315697No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.15539728054759042No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.15539728054759042No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.15539728054759042No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA840.15539728054759042No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA820.15169734529645731No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA810.14984737767089076No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA790.14614744241975766No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA790.14614744241975766No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.14429747479419108No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.14244750716862453No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA750.13874757191749146No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA750.13874757191749146No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA740.13689760429192488No Hit
GCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA740.13689760429192488No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.13504763666635833No Hit
CCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA720.13319766904079178No Hit
ACCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA710.13134770141522523No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.13134770141522523No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA710.13134770141522523No Hit
GCCTACGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA700.1294977337896587No Hit
TCCTACGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA700.1294977337896587No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA700.1294977337896587No Hit
TCCTACGGGGTGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA690.12764776616409212No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA690.12764776616409212No Hit
GCCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA690.12764776616409212No Hit
TCCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA680.12579779853852557No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.12579779853852557No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA660.12209786328739247No Hit
TCCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA650.12024789566182591No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.12024789566182591No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA620.11469799278512627No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA610.1128480251595597No Hit
ACCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA610.1128480251595597No Hit
GCCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA600.11099805753399315No Hit
ACCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA600.11099805753399315No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA600.11099805753399315No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.10914808990842659No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.10729812228286006No Hit
TCCTATGGGGCGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA570.1054481546572935No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA560.10359818703172695No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.10359818703172695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)