FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32227
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1040.3227107704719645No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1030.31960778229434944No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.3165047941167344No Hit
AGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA910.28237192416296897No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA900.2792689359853539No Hit
CGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA880.2730629596301238No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA870.2699599714525088No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA840.26065100691966364No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA820.25444503056443357No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.2513420423868185No Hit
TGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA790.2451360660315884No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.2451360660315884No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.24203307785397335No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.2296211251435132No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.22031216061066805No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA670.2079002079002079No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA660.20479721972259285No Hit
TGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA650.20169423154497781No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.20169423154497781No Hit
TGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA640.19859124336736278No Hit
AGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA630.19548825518974772No Hit
AGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA630.19548825518974772No Hit
AGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA630.19548825518974772No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.19238526701213268No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA610.18928227883451765No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.18928227883451765No Hit
CGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA580.17997331430167252No Hit
CGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA580.17997331430167252No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA570.17687032612405748No Hit
TGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA560.17376733794644242No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.17376733794644242No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA550.17066434976882738No Hit
CGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA550.17066434976882738No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA540.16756136159121235No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA530.1644583734135973No Hit
TGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA530.1644583734135973No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA510.1582523970583672No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.15514940888075218No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA490.15204642070313712No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA490.15204642070313712No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA490.15204642070313712No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA480.14894343252552208No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA480.14894343252552208No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA480.14894343252552208No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA470.14584044434790702No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA470.14584044434790702No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA470.14584044434790702No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA460.142737456170292No Hit
TGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA460.142737456170292No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA460.142737456170292No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA450.13963446799267695No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.13963446799267695No Hit
TGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA450.13963446799267695No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.13963446799267695No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA450.13963446799267695No Hit
TGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA440.1365314798150619No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.1365314798150619No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA440.1365314798150619No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA440.1365314798150619No Hit
TGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA440.1365314798150619No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA440.1365314798150619No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA440.1365314798150619No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.13342849163744686No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA430.13342849163744686No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA430.13342849163744686No Hit
AGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA420.13032550345983182No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.13032550345983182No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA420.13032550345983182No Hit
TGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA420.13032550345983182No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.13032550345983182No Hit
TGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA420.13032550345983182No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA410.12722251528221679No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA410.12722251528221679No Hit
TGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA410.12722251528221679No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA410.12722251528221679No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA410.12722251528221679No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA400.12411952710460172No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.12411952710460172No Hit
TGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA400.12411952710460172No Hit
CGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA390.12101653892698667No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTTCTCA390.12101653892698667No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA380.11791355074937165No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA380.11791355074937165No Hit
TGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA380.11791355074937165No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA380.11791355074937165No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA380.11791355074937165No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA380.11791355074937165No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA380.11791355074937165No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA380.11791355074937165No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA380.11791355074937165No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA370.1148105625717566No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA370.1148105625717566No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA370.1148105625717566No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA370.1148105625717566No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTTCTCA370.1148105625717566No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA370.1148105625717566No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA370.1148105625717566No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTTCTCA360.11170757439414156No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTTCTCA360.11170757439414156No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA360.11170757439414156No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA360.11170757439414156No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA360.11170757439414156No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA360.11170757439414156No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA360.11170757439414156No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA360.11170757439414156No Hit
TGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA360.11170757439414156No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA350.10860458621652651No Hit
AGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA350.10860458621652651No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA350.10860458621652651No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA350.10860458621652651No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA350.10860458621652651No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA350.10860458621652651No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA350.10860458621652651No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA350.10860458621652651No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA350.10860458621652651No Hit
TGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA340.10550159803891149No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA340.10550159803891149No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA340.10550159803891149No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA340.10550159803891149No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA340.10550159803891149No Hit
AGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA340.10550159803891149No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA340.10550159803891149No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA340.10550159803891149No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA330.10239860986129642No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA330.10239860986129642No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA330.10239860986129642No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA330.10239860986129642No Hit
AGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA330.10239860986129642No Hit
TGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA330.10239860986129642No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTTCTCA330.10239860986129642No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA330.10239860986129642No Hit
CGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCA330.10239860986129642No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA330.10239860986129642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)