FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43198
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA16293.7710079170331956No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA7381.7084124265012268No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6271.4514560859299042No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4461.0324552062595491No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4070.9421732487615168No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4050.9375434047872586No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3900.9028195749803231No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3820.8843001990832908No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3480.8055928515209038No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3260.754664567804065No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3160.7315153479327746No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3080.7129959720357424No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3000.6944765961387102No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2980.689846752164452No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2840.6574378443446456No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2780.6435483124218714No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2680.620399092550581No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2650.613454326589194No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2630.6088244826149358No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA2630.6088244826149358No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2610.6041946386406778No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2560.5926200287050326No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2470.5717857308208714No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2430.5625260428723552No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2430.5625260428723552No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2420.5602111208852262No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2380.55095143293671No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2340.541691744988194No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2270.5254872910782906No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2250.5208574471040326No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA2220.5139126811426454No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2160.5000231492198713No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2140.4953933052456132No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2120.4907634612713552No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2060.47687392934858097No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2040.47224408537432283No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1980.4583545534515487No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1930.44677994351590355No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1920.4444650215287745No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1910.4421500995416454No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1850.42826056761887127No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1830.4236307236446132No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1830.4236307236446132No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.412056113708968No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.412056113708968No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1710.3958516597990648No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.39353673781193577No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1690.3912218158248067No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1650.3819621278762906No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1640.37964720588916157No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1540.3564979860178712No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1530.3541830640307422No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1520.3518681420436131No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1510.3495532200564841No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1490.344923376082226No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.342608454095097No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1470.340293532107968No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1450.3356636881337099No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.3310338441594518No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1430.3310338441594518No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1410.3264040001851938No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1370.31714431223667766No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1370.31714431223667766No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1370.31714431223667766No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1360.3148293902495486No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.2916801703782583No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1240.2870503264040002No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1230.2847354044168712No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1230.2847354044168712No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1200.277790638455484No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1190.275475716468355No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1150.2662160285198389No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1130.2615861845455808No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1120.2592712625584518No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.2476966526228066No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1060.2453817306356776No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA1010.23380712070003243No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1000.23149219871290336No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA980.2268623547386453No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA980.2268623547386453No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA960.22223251076438724No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA930.21528774480300011No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA880.20371313486735498No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA870.20139821288022594No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA860.19908329089309693No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.19445344691883884No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.19445344691883884No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA840.19445344691883884No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA830.1921385249317098No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA830.1921385249317098No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA810.18750868095745174No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA810.18750868095745174No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA800.1851937589703227No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA790.18287883698319365No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA720.16667438307329044No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA710.1643594610861614No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA710.1643594610861614No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA700.16204453909903235No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA700.16204453909903235No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.1597296171119033No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA680.1574146951247743No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA670.15509977313764525No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA660.15278485115051624No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA660.15278485115051624No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA660.15278485115051624No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.1435251632020001No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.14121024121487105No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGA590.136580397240613No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.136580397240613No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA580.13426547525348395No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA580.13426547525348395No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.13426547525348395No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.13426547525348395No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA570.13195055326635494No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.1296356312792259No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.1296356312792259No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA550.12732070929209685No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA550.12732070929209685No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA550.12732070929209685No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA540.12500578730496784No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA540.12500578730496784No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA530.1226908653178388No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA530.1226908653178388No Hit
GCCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA530.1226908653178388No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA530.1226908653178388No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.12037594333070975No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.12037594333070975No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.12037594333070975No Hit
TCCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA510.11806102134358071No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.11574609935645168No Hit
ACCTATGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA500.11574609935645168No Hit
TCCTACGGGGTGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA500.11574609935645168No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA490.11343117736932265No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA480.11111625538219362No Hit
GCCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA480.11111625538219362No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA480.11111625538219362No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA480.11111625538219362No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA480.11111625538219362No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA470.10880133339506459No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA470.10880133339506459No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA470.10880133339506459No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA470.10880133339506459No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.10880133339506459No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA460.10648641140793555No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA460.10648641140793555No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGGAGTCTGA460.10648641140793555No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA460.10648641140793555No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA450.10417148942080652No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA450.10417148942080652No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA450.10417148942080652No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA440.10185656743367749No Hit
ACCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA440.10185656743367749No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.10185656743367749No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA440.10185656743367749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)