FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15380
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5393.5045513654096228No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2981.9375812743823149No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2161.4044213263979195No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1440.9362808842652794No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1390.9037711313394018No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1390.9037711313394018No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1380.8972691807542262No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1230.799739921976593No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1220.7932379713914174No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1170.7607282184655397No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1150.7477243172951885No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1120.7282184655396619No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1090.7087126137841352No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1030.6697009102730819No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA960.6241872561768531No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.6241872561768531No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA940.6111833550065019No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.6046814044213265No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.5981794538361509No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA890.5786736020806242No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA880.5721716514954486No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA870.5656697009102731No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.5591677503250976No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.5461638491547465No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.5461638491547465No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.5396618985695709No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA820.5331599479843953No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA810.5266579973992198No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA790.5136540962288686No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA790.5136540962288686No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.5071521456436932No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.5071521456436932No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.5071521456436932No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA750.4876462938881665No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.4616384915474643No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.45513654096228867No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.45513654096228867No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.45513654096228867No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA680.4421326397919376No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA660.42912873862158646No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.42912873862158646No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.42912873862158646No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.42262678803641096No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.42262678803641096No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA640.41612483745123535No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA640.41612483745123535No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA640.41612483745123535No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA640.41612483745123535No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA620.4031209362808843No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA600.3901170351105332No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA600.3901170351105332No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA600.3901170351105332No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.3836150845253576No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.37711313394018203No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.37711313394018203No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.37061118335500653No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA560.3641092327698309No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA550.3576072821846554No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.3576072821846554No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.34460338101430427No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.34460338101430427No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA520.3381014304291287No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA520.3381014304291287No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.3315994798439532No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.3315994798439532No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.3315994798439532No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA500.3250975292587776No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.3250975292587776No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.3185955786736021No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA480.31209362808842656No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA460.29908972691807545No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.29908972691807545No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.29908972691807545No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.2925877763328999No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA450.2925877763328999No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.2795838751625488No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA430.2795838751625488No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA420.27308192457737324No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA400.26007802340702213No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA390.2535760728218466No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA380.247074122236671No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA380.247074122236671No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA380.247074122236671No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.247074122236671No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.24057217165149547No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA370.24057217165149547No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA370.24057217165149547No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGA360.23407022106631986No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA360.23407022106631986No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA360.23407022106631986No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA350.22756827048114434No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA340.2210663198959688No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA340.2210663198959688No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA330.21456436931079323No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA330.21456436931079323No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.1950585175552666No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA290.18855656697009102No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA280.18205461638491546No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA280.18205461638491546No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA280.18205461638491546No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA280.18205461638491546No Hit
GCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA270.17555266579973994No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA270.17555266579973994No Hit
CCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA260.16905071521456436No Hit
GCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA250.1625487646293888No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA250.1625487646293888No Hit
CCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA250.1625487646293888No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA250.1625487646293888No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA240.15604681404421328No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA240.15604681404421328No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA240.15604681404421328No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA240.15604681404421328No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA230.14954486345903772No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA230.14954486345903772No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA220.14304291287386214No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA220.14304291287386214No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA220.14304291287386214No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA220.14304291287386214No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA220.14304291287386214No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA220.14304291287386214No Hit
CCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA220.14304291287386214No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA220.14304291287386214No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA220.14304291287386214No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA210.13654096228868662No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA210.13654096228868662No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA210.13654096228868662No Hit
ACCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA210.13654096228868662No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA210.13654096228868662No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA200.13003901170351106No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA200.13003901170351106No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA200.13003901170351106No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA200.13003901170351106No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA190.1235370611183355No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA190.1235370611183355No Hit
CCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA190.1235370611183355No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA190.1235370611183355No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA190.1235370611183355No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA180.11703511053315993No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA180.11703511053315993No Hit
ACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA180.11703511053315993No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA180.11703511053315993No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA180.11703511053315993No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA180.11703511053315993No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA170.1105331599479844No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA170.1105331599479844No Hit
TCCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA170.1105331599479844No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGA170.1105331599479844No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA170.1105331599479844No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA170.1105331599479844No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA170.1105331599479844No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA160.10403120936280884No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA160.10403120936280884No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA160.10403120936280884No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA160.10403120936280884No Hit
TCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA160.10403120936280884No Hit
GCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA160.10403120936280884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)