FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29134
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA13194.527356353401524No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5121.7573968559071875No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3901.338642136335553No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3721.2768586531200659No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3721.2768586531200659No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3551.2185075856387726No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3501.2013455069678038No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3301.1326971922839295No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3161.0846433720052173No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2810.964508821308437No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2720.9336170797006934No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2700.9267522482323058No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2690.9233198324981121No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2460.8443742706116565No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2430.8340770234090753No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2340.8031852818013318No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2300.7894556188645568No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2290.7860232031303631No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2290.7860232031303631No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2290.7860232031303631No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2260.775725955927782No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2230.7654287087252007No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2210.7585638772568133No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2160.7414017985858448No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2130.7311045513832636No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2120.7276721356490699No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2120.7276721356490699No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1980.6796183153703577No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1950.6693210681677765No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1830.6281320793574517No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.6109700006864832No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1720.5903755062813207No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.5354568545342212No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1550.5320244388000275No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.52515960733164No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1510.5182947758632526No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.5148623601290588No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.5148623601290588No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.5148623601290588No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.49083544998970274No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA1410.4839706185213153No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1390.47710578705292783No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1340.45994370838195925No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1310.449646461179378No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1090.3741333150271161No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1040.3569712363561474No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA890.3054850003432416No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA870.2986201688748541No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA820.28145809020388546No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA800.27459325873549806No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.2677284272671106No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.25056634859614196No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA690.23683668565936708No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA670.22997185419097962No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA640.21967460698839844No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.21280977552001096No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA600.2059449440516235No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA600.2059449440516235No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA590.20251252831742983No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA540.1853504496464612No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA540.1853504496464612No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.18191803391226746No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA520.1784856181780737No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.17505320244388No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.17162078670968628No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.16818837097549255No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA490.16818837097549255No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA480.16475595524129882No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA480.16475595524129882No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.1613235395071051No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA470.1613235395071051No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA450.15445870803871767No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA450.15445870803871767No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA450.15445870803871767No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.15445870803871767No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.1510262923045239No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.14759387657033018No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA430.14759387657033018No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA420.14416146083613646No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA410.14072904510194273No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA410.14072904510194273No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA400.13729662936774903No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA390.1338642136335553No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA390.1338642136335553No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA390.1338642136335553No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA380.13043179789936157No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA380.13043179789936157No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.12699938216516785No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.12699938216516785No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA360.12356696643097412No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA360.12356696643097412No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA360.12356696643097412No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA360.12356696643097412No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA360.12356696643097412No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA360.12356696643097412No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA350.1201345506967804No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGGGAAACCCTGA350.1201345506967804No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA350.1201345506967804No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA350.1201345506967804No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA350.1201345506967804No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA340.11670213496258668No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA340.11670213496258668No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA340.11670213496258668No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.11326971922839295No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.11326971922839295No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA320.10983730349419922No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA320.10983730349419922No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA320.10983730349419922No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA320.10983730349419922No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA310.10640488776000548No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.10640488776000548No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA310.10640488776000548No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.10640488776000548No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA300.10297247202581175No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.10297247202581175No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.10297247202581175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)