FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15822
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7194.544305397547718No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2471.5611174314245988No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2141.3525470863354823No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1991.2577423840222475No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1921.2135001896094046No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1911.2071798761218557No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1671.05549235242068No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1550.9796485905700922No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.9670079635949944No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.9354063961572494No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.9290860826697004No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1410.8911642017444065No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1400.8848438882568576No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1330.8406016938440147No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1320.8342813803564657No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1220.7710782454809759No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.7647579319934269No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.7457969915307799No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1160.733156364555682No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1150.726836051068133No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1090.6889141701428391No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.6762735431677411No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1040.6573126027050942No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.6509922892175452No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1020.6446719757299962No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1000.6320313487548982No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA980.6193907217798003No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA970.6130704082922513No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.6067500948047023No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.5877891543420554No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.5814688408545065No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.5751485273669574No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.5751485273669574No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA890.5625079003918595No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA820.5182657059790166No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA820.5182657059790166No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA790.49930476551636965No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA740.4677031980786247No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA740.4677031980786247No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA710.44874225761597775No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.44874225761597775No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA690.4361016306408798No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA690.4361016306408798No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA670.4234610036657818No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA670.4234610036657818No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA670.4234610036657818No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA660.41714069017823285No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.41714069017823285No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA590.37289849576538997No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA580.366578182277841No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA560.353937555302743No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA550.34761724181519404No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA540.3412969283276451No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA520.3286563013525471No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.3223359878649981No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA510.3223359878649981No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA500.3160156743774491No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA490.30969536088990013No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA490.30969536088990013No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA460.2907344204272532No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA450.2844141069397042No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA450.2844141069397042No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.2844141069397042No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA440.27809379345215524No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA440.27809379345215524No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA430.2717734799646062No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA420.26545316647705725No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.2528125395019593No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.2528125395019593No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.2528125395019593No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA400.2528125395019593No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.2528125395019593No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA370.23385159903931235No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA340.2148906585766654No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA330.20857034508911643No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA330.20857034508911643No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA330.20857034508911643No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA330.20857034508911643No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA330.20857034508911643No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA310.19592971811401844No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA300.18960940462646947No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA300.18960940462646947No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA300.18960940462646947No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA300.18960940462646947No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.1832890911389205No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.1832890911389205No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA280.1769687776513715No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA280.1769687776513715No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.17064846416382254No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.17064846416382254No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA260.16432815067627354No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA240.15168752370117558No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA240.15168752370117558No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA240.15168752370117558No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA230.1453672102136266No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA230.1453672102136266No Hit
ACCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA230.1453672102136266No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA220.13904689672607762No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA210.13272658323852862No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA210.13272658323852862No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGA210.13272658323852862No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA210.13272658323852862No Hit
TCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA200.12640626975097966No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA200.12640626975097966No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.12640626975097966No Hit
CCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA200.12640626975097966No Hit
CCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA200.12640626975097966No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA190.12008595626343066No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA180.11376564277588168No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA180.11376564277588168No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA180.11376564277588168No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA180.11376564277588168No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA180.11376564277588168No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA180.11376564277588168No Hit
GCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA180.11376564277588168No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA180.11376564277588168No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA180.11376564277588168No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA170.1074453292883327No Hit
GCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA170.1074453292883327No Hit
CCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA170.1074453292883327No Hit
CCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA170.1074453292883327No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA170.1074453292883327No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGA170.1074453292883327No Hit
CCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA170.1074453292883327No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA170.1074453292883327No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA170.1074453292883327No Hit
CCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA160.10112501580078373No Hit
ACCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA160.10112501580078373No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA160.10112501580078373No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA160.10112501580078373No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA160.10112501580078373No Hit
ACCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA160.10112501580078373No Hit
ACCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA160.10112501580078373No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA160.10112501580078373No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA160.10112501580078373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)