FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29937
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA14304.776697731903664No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5841.9507632695326853No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4531.5131776731135385No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4191.3996058389284163No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4111.3728830544142698No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3951.3194374853859774No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA3891.2993953970003673No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3871.292714700871831No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3151.0522096402445134No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3091.0321675518589037No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3081.0288272037946353No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3041.0154658115375623No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3031.012125463473294No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA3031.012125463473294No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3011.0054447673447573No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2930.978721982830611No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2890.9653605905735378No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2800.9352974579951231No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2760.9219360657380499No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2720.9085746734809768No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2580.8618098005812206No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2460.8217256238100009No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2390.7983431873601229No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2370.7916624912315864No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.7783010989745132No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2250.7515783144603667No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2190.731536226074757No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2120.708153789624879No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2080.6947923973678057No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2070.6914520493035374No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1970.6580485686608545No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1940.6480275244680496No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1860.6213047399539032No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1820.60794334769683No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.5912416073754885No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1740.5812205631826837No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1730.5778802151184154No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1720.5745398670541471No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1710.5711995189898788No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.5210942980258543No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1550.517753949961586No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1540.5144136018973177No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.4876908173831713No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1400.46764872899756155No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1300.4342452483548786No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.36743828706951265No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1100.36743828706951265No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1070.35741724287670773No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1010.33737515449109795No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1010.33737515449109795No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1000.3340348064268297No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA1000.3340348064268297No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA980.3273541102982931No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA980.3273541102982931No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA970.3240137622340248No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA970.3240137622340248No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA950.3173330661054882No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGA940.31399271804121986No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA930.3106523699769516No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA920.3073120219126833No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA870.29061028159134183No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA850.2839295854628052No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA840.2805892373985369No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA820.27390854127000036No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA820.27390854127000036No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA820.27390854127000036No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA810.27056819320573205No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA790.26388749707719544No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA780.26054714901292714No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA780.26054714901292714No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA740.24718575675585397No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA730.24384540869158566No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.24384540869158566No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA720.24050506062731739No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA720.24050506062731739No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.23716471256304905No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA710.23716471256304905No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.23716471256304905No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA700.23382436449878077No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA690.23048401643451247No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA670.22380332030597586No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA660.22046297224170758No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.2171226241774393No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA650.2171226241774393No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.2171226241774393No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA630.21044192804890272No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA630.21044192804890272No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA610.2037612319203661No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA610.2037612319203661No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA600.2004208838560978No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.19708053579182952No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA580.1937401877275612No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA570.19039983966329294No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.19039983966329294No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA560.18705949159902463No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA550.18371914353475632No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA550.18371914353475632No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA550.18371914353475632No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA540.18037879547048802No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA540.18037879547048802No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA530.17703844740621974No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA530.17703844740621974No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA530.17703844740621974No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.17703844740621974No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA530.17703844740621974No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.17703844740621974No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA520.17369809934195143No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA520.17369809934195143No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.17035775127768313No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.17035775127768313No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA510.17035775127768313No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.16701740321341485No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA480.16033670708487824No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.15365601095634165No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA450.15031566289207335No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA450.15031566289207335No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA450.15031566289207335No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA450.15031566289207335No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA430.14363496676353676No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.14363496676353676No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA420.14029461869926846No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA400.13361392257073187No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA400.13361392257073187No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA400.13361392257073187No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGA390.13027357450646357No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGA390.13027357450646357No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA390.13027357450646357No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA390.13027357450646357No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.13027357450646357No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.12359287837792698No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA370.12359287837792698No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA360.12025253031365869No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA350.11691218224939039No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.1135718341851221No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGA340.1135718341851221No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGA340.1135718341851221No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA340.1135718341851221No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.11023148612085379No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA330.11023148612085379No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA320.1068911380565855No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA320.1068911380565855No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA320.1068911380565855No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.1068911380565855No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.1068911380565855No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA320.1068911380565855No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA320.1068911380565855No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA310.10355078999231719No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA310.10355078999231719No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGA300.1002104419280489No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.1002104419280489No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA300.1002104419280489No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.1002104419280489No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA300.1002104419280489No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA300.1002104419280489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)