FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45199
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA19394.289917918538021No Hit
GCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA11362.5133299409278966No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6821.5088829398880506No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5081.1239186707670523No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4781.0575455209186044No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4541.0044470010398459No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4450.9845350560853117No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4350.9624106728024957No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4320.9557733578176509No Hit
TCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4290.949136042832806No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4020.8894002079692029No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3660.8097524281510653No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3550.7854156065399677No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3510.7765658532268414No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3410.7544414699440253No Hit
TCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3180.7035553883935486No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3170.7013429500652669No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3150.6969180734087037No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3060.6770061284541693No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3040.6725812517976062No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2940.6504568685147901No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2920.6460319918582269No Hit
TCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2910.6438195535299454No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2840.6283324852319742No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2780.6150578552622845No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2760.6106329786057214No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2670.590721033651187No Hit
TCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2660.5885085953229053No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2630.5818712803380606No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2620.579658842009779No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2590.5730215270249341No Hit
GCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2500.5531095820703997No Hit
ACCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2500.5531095820703997No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2450.5420473904289917No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2420.535410075444147No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.5331976371158653No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2300.5088608155047678No Hit
GCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2270.5022235005199229No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2260.5000110621916414No Hit
ACCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2200.48673643222195184No Hit
GCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2160.47788667890882547No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2150.4756742405805438No Hit
ACCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2140.4734618022522622No Hit
TCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1980.4380627889997567No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1940.4292130356866302No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1930.42700059735834867No Hit
TCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1920.42478815903006706No Hit
TCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1920.42478815903006706No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1910.42257572070178545No Hit
GCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1910.42257572070178545No Hit
ACCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1900.42036328237350384No Hit
TCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1880.4159384057169407No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1870.4137259673886591No Hit
CCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1830.40487621407553265No Hit
ACCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1810.40045133741896943No Hit
GCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1790.3960264607624063No Hit
CCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1780.39381402243412467No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.39381402243412467No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.39160158410584306No Hit
GCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1770.39160158410584306No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.38717670744927984No Hit
TCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1750.38717670744927984No Hit
ACCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1730.3827518307927167No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.37168963915130865No Hit
CCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1650.3650523241664638No Hit
TCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1580.34956525586849263No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1570.3473528175402111No Hit
ACCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1560.34514037921192947No Hit
GCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1500.3318657492422399No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1490.3296533109139583No Hit
CCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1490.3296533109139583No Hit
CCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1460.32301599592911345No Hit
ACCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1410.31195380428770547No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.2964667359897343No Hit
CCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1340.2964667359897343No Hit
GCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1320.29204185933317106No Hit
ACCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1320.29204185933317106No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1300.2876169826766079No Hit
CCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1240.2743423527069183No Hit
CCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1210.2677050377220735No Hit
CCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1190.2632801610655103No Hit
GCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1160.2566428460806655No Hit
CCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1130.2500055310958207No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1100.24336821611097592No Hit
TCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1080.23894333945441273No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1040.2300935861412863No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1040.2300935861412863No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA980.21681895617159672No Hit
ACCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA960.21239407951503353No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA940.20796920285847034No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA930.2057567645301887No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA930.2057567645301887No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA860.1902696962322175No Hit
ACCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA820.18141994291909114No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA800.17699506626252792No Hit
GCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA780.17257018960596474No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA750.16593287462111994No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA720.15929555963627515No Hit
CCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA720.15929555963627515No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA700.15487068297971193No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA690.15265824465143035No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA680.15044580632314875No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA650.14380849133830395No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGGGAAACCCTGA640.14159605301002234No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA630.13938361468174074No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGA600.13274629969689594No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.12832142304033275No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA580.12832142304033275No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA560.12389654638376955No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA550.12168410805548796No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.11947166972720637No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.11725923139892475No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA520.11504679307064315No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA510.11283435474236156No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.11062191641407995No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.10840947808579836No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.10840947808579836No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.10619703975751676No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.10398460142923517No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGA460.10177216310095358No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.10177216310095358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)