FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35839
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA18155.064315410586233No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6391.782973855297302No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4951.3811769301598817No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4771.3309523145177042No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA4681.3058400066966154No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4651.2974692374229193No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4261.188649236864868No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4081.1384246212226903No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4071.135634364798125No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4041.1272635955244288No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3711.0351851335137698No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3270.9124138508328916No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3190.8900917994363681No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3110.8677697480398449No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3090.862189235190714No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3070.8566087223415833No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2970.828706158095929No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2960.8259159016713636No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2800.7812717988783169No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA2780.775691286029186No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2770.7729010296046206No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2770.7729010296046206No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2760.7701107731800553No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2670.7449984653589665No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2660.7422082089344011No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2530.7059348754150507No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2480.6919835932922235No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2480.6919835932922235No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.6724517983202657No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2350.6557102597728731No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2340.6529200033483077No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2090.5831635927341723No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2060.574792823460476No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2030.5664220541867797No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2010.560841541337649No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1840.5134071821200369No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1800.5022461564217752No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1800.5022461564217752No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1790.4994558999972098No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1690.47155333575155556No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1640.45760205362872847No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1620.45202154077959766No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1600.4464410279304668No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.4185384636848127No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.4185384636848127No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1380.38505538659002764No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1350.3766846173163314No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1340.373894360891766No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1340.373894360891766No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1270.354362565919808No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1220.34041128379698093No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1220.34041128379698093No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1160.3236697452495884No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA1130.3152989759758922No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1120.3125087195513268No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1030.287396411730238No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.287396411730238No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA980.27344512960741096No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA980.27344512960741096No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA960.26786461675828005No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA920.25670359106001844No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA870.2427523089371913No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA860.23996205251262592No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA850.2371717960880605No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA850.2371717960880605No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA830.23159128323892966No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA830.23159128323892966No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA820.22880102681436423No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA810.22601077038979883No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGA800.2232205139652334No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA790.22043025754066797No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA790.22043025754066797No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA790.22043025754066797No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA780.2176400011161026No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA770.21484974469153714No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA760.2120594882669717No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA760.2120594882669717No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA750.20926923184240634No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA740.2064789754178409No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA730.20368871899327545No Hit
ACCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA710.19810820614414465No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA690.19252769329501382No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA690.19252769329501382No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA670.186947180445883No Hit
TCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA660.18415692402131756No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA660.18415692402131756No Hit
GCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA660.18415692402131756No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA650.18136666759675213No Hit
TCCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA640.17857641117218673No Hit
ACCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA640.17857641117218673No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.17857641117218673No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.17578615474762133No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA630.17578615474762133No Hit
TCCTACGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA620.17299589832305587No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA620.17299589832305587No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA600.16741538547392507No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.16741538547392507No Hit
GCCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA590.16462512904935964No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA570.1590446162002288No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA570.1590446162002288No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA570.1590446162002288No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA570.1590446162002288No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA560.1562543597756634No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA540.15067384692653255No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.15067384692653255No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.15067384692653255No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA530.14788359050196714No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.14788359050196714No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA530.14788359050196714No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.14788359050196714No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.14788359050196714No Hit
ACCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA520.14509333407740171No Hit
ACCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA520.14509333407740171No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.14509333407740171No Hit
GCCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA510.14230307765283629No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA500.13951282122827088No Hit
TCCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA500.13951282122827088No Hit
CCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA500.13951282122827088No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.13951282122827088No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.13672256480370548No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA490.13672256480370548No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.13672256480370548No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.13672256480370548No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA490.13672256480370548No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA490.13672256480370548No Hit
TCCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA480.13393230837914002No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.13114205195457462No Hit
CCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA470.13114205195457462No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.12835179553000922No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.12835179553000922No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.12835179553000922No Hit
ACCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA460.12835179553000922No Hit
GCCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA460.12835179553000922No Hit
GCCTACGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA450.1255615391054438No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA450.1255615391054438No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA450.1255615391054438No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA450.1255615391054438No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.12277128268087838No Hit
CCCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA440.12277128268087838No Hit
TCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA440.12277128268087838No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA430.11998102625631296No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.11998102625631296No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA420.11719076983174753No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA420.11719076983174753No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.11719076983174753No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.11719076983174753No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.11440051340718212No Hit
TCCTACGGGGTGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA410.11440051340718212No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.11440051340718212No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA410.11440051340718212No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.11440051340718212No Hit
TCCTATGGGGCGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA400.1116102569826167No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.1116102569826167No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.1116102569826167No Hit
TCCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA400.1116102569826167No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.1088200005580513No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA390.1088200005580513No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA390.1088200005580513No Hit
CCCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA390.1088200005580513No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA380.10602974413348586No Hit
ACCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA380.10602974413348586No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.10602974413348586No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.10323948770892045No Hit
ACCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA370.10323948770892045No Hit
ACCTACGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA370.10323948770892045No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.10323948770892045No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.10044923128435504No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA360.10044923128435504No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA360.10044923128435504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)