FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36802
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA26277.13819901092332No Hit
TCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA9792.6601815118743546No Hit
GCCTACGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA8592.3341122765067115No Hit
TCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA6811.8504429107113745No Hit
TCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA6741.8314222053149285No Hit
GCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA6471.7580566273572087No Hit
ACCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA6341.7227324601923808No Hit
GCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA6091.6548013694907886No Hit
TCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA6001.6303461768382155No Hit
ACCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA5651.5352426498559861No Hit
ACCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA5501.4944839954350306No Hit
TCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA5261.429270148361502No Hit
GCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA5171.404814955708929No Hit
TCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4741.28797347970219No Hit
GCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4721.2825389924460628No Hit
CCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4561.2390630943970435No Hit
ACCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4541.2336286071409162No Hit
TCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4481.2173251453725342No Hit
TCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4451.209173414488343No Hit
TCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4451.209173414488343No Hit
GCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4411.1983044399760883No Hit
GCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4381.1901527090918973No Hit
CCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4251.1548285419270692No Hit
ACCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4221.1466768110428782No Hit
CCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4091.11135264387805No Hit
ACCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3701.005380142383566No Hit
ACCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3670.997228411499375No Hit
GCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3660.9945111678713113No Hit
CCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3580.9727732188468018No Hit
CCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3510.9537525134503559No Hit
TCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3460.9401662953100375No Hit
GCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3230.8776696918645726No Hit
GCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3060.8314765501874898No Hit
TCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3040.8260420629313625No Hit
ACCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2930.7961523830226618No Hit
TCCTATGGGGCGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA2880.7825661648823433No Hit
CCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2790.7581109722297702No Hit
CCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2780.7553937286017064No Hit
CCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2770.7526764849736427No Hit
CCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2740.7445247540894517No Hit
ACCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2610.7092005869246237No Hit
ACCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2550.6928971251562415No Hit
GCCTATGGGGCGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA2400.6521384707352861No Hit
CCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2300.6249660344546492No Hit
TCCTACGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA2290.6222487908265856No Hit
TCCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA2250.6113798163143307No Hit
ACCTATGGGGCGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA2140.5814901364056302No Hit
GCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2080.5651866746372479No Hit
TCCTACGGGGTGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1880.5108418020759741No Hit
ACCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1820.49453834030759203No Hit
ACCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1760.4782348785392098No Hit
TCCTACGGGGCGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1750.47551763491114607No Hit
GCCTACGGGGCGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1750.47551763491114607No Hit
GCCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1730.47008314765501874No Hit
TCCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1730.47008314765501874No Hit
ACCTACGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1650.44834519863050915No Hit
CCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1640.4456279550024455No Hit
ACCTACGGGGCGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1520.41302103146568125No Hit
CCCTATGGGGCGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1500.40758654420955387No Hit
TCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1460.39671756969729904No Hit
GCCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1390.3776968643008532No Hit
TCCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1390.3776968643008532No Hit
CCCTACGGGAGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1340.36411064616053473No Hit
GCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1330.3613934025324711No Hit
TCCTATGGGGCGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1320.3586761589044074No Hit
ACCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1310.35595891527634366No Hit
ACCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1250.3396554535079615No Hit
CCCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1250.3396554535079615No Hit
ACCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1200.32606923536764304No Hit
GCCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1160.31520026085538827No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1160.31520026085538827No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA1140.3097657735992609No Hit
GCCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1140.3097657735992609No Hit
CCCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1090.2961795554589424No Hit
TCCTACGGGGCGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1090.2961795554589424No Hit
CCCTACGGGGCGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1070.2907450682028151No Hit
ACCTATGGGGCGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA1040.28259333731862396No Hit
CCCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA990.2690071191783055No Hit
CCCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA990.2690071191783055No Hit
CCCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA970.26357263192217817No Hit
ACCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA940.25542090103798704No Hit
ACCTACGGGGTGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA880.2391174392696049No Hit
CCCTACGGGGTGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA880.2391174392696049No Hit
GCCTACGGGAGGCTGTAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA860.23368295201347755No Hit
TCCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA830.22553122112928645No Hit
GCCTACGGGGCGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA830.22553122112928645No Hit
GCCTATGGGGCGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA820.22281397750122275No Hit
ACCTACGGGGCGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA770.2092277593609043No Hit
GCCTACGGGGTGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA690.18748981033639478No Hit
CCCTATGGGGCGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA670.18205532308026737No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA570.15488288679963044No Hit
GCCTACGGGAGGCTGCAGCTGCGAATCTTCCACAATGGACGAAAGTCTGA500.1358621814031846No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.12771045051899352No Hit
CCCTACGGGGCGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGA460.12499320689092984No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA430.11684147600673878No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA430.11684147600673878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)