FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18824
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9334.956438589035274No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA3751.9921376965575859No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3441.827454313642159No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2351.248406289842754No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2231.1846578835529111No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2231.1846578835529111No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2211.1740331491712708No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2191.1634084147896302No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2001.062473438164046No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.9615384615384616No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1740.92435189120272No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1710.9084147896302592No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.9031024224394391No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1580.8393540161495963No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1570.8340416489587761No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.8287292817679558No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.8287292817679558No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.8127921801954952No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.7968550786230344No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.7756056098597536No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1450.7702932426689333No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.7596685082872928No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1380.7331066723331917No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.7277943051423714No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1370.7277943051423714No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.7224819379515512No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.7118572035699108No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1280.6799830004249894No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1250.6640458988525287No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.6587335316617084No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1190.6321716957076072No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1130.6002974925626859No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5684232894177645No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5684232894177645No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1060.5631109222269443No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.5631109222269443No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1010.5365490862728431No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.5365490862728431No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA990.5259243518912027No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.509987250318742No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.509987250318742No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA900.47811304717382064No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA900.47811304717382064No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA900.47811304717382064No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA880.46748831279218017No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA860.45686357841053976No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA840.4462388440288993No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA840.4462388440288993No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.44092647683807906No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA820.4356141096472589No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA810.43030174245643854No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA760.40373990650233743No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA750.3984275393115172No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA720.3824904377390565No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA710.37717807054823627No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA710.37717807054823627No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA690.36655333616659586No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA690.36655333616659586No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA680.3612409689757756No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA670.3559286017849554No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA670.3559286017849554No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA670.3559286017849554No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA660.3506162345941351No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA620.3293667658308542No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.3293667658308542No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA600.3187420314492138No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA600.3187420314492138No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA590.3134296642583935No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA580.3081172970675733No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA580.3081172970675733No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA580.3081172970675733No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA560.2974925626859329No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA550.29218019549511265No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA550.29218019549511265No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA540.2868678283042924No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA540.2868678283042924No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.2815554611134721No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA510.2709307267318317No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA500.2656183595410115No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA490.26030599235019125No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.24968125796855076No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA460.24436889077773055No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA450.23905652358691032No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA440.23374415639609009No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA440.23374415639609009No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA430.22843178920526988No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA430.22843178920526988No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA420.22311942201444965No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA390.20718232044198895No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA380.20186995325116872No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA380.20186995325116872No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA380.20186995325116872No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA380.20186995325116872No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA350.18593285167870802No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA350.18593285167870802No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA330.17530811729706755No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA330.17530811729706755No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA310.1646833829154271No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA290.15405864853378665No Hit
ACCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA280.14874628134296644No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGGGAAACCCTGA270.1434339141521462No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA270.1434339141521462No Hit
TCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA260.13812154696132595No Hit
GCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA250.13280917977050574No Hit
TCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA250.13280917977050574No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA250.13280917977050574No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA240.1274968125796855No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.1274968125796855No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA240.1274968125796855No Hit
GCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA230.12218444538886528No Hit
GCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA230.12218444538886528No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA220.11687207819804504No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA210.11155971100722482No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGA210.11155971100722482No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA210.11155971100722482No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA210.11155971100722482No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA200.10624734381640458No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA190.10093497662558436No Hit
TCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA190.10093497662558436No Hit
CCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA190.10093497662558436No Hit
ACCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA190.10093497662558436No Hit
ACCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA190.10093497662558436No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA190.10093497662558436No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA190.10093497662558436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)