FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19883
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA10265.160187094502842No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4112.067092491072776No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA2641.327767439521199No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2631.3227380174018006No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2561.2875320625660112No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2531.2724437962078157No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2501.2573555298496204No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2501.2573555298496204No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2221.1165317105064627No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2111.0612080671930795No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2101.056178645073681No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1991.0008550017602977No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1910.96061962480511No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.9103254036111251No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1790.900266559372328No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1790.900266559372328No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.8952371372529296No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1730.8700900266559373No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1690.8499723381783434No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1690.8499723381783434No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.8449429160589448No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1620.814766383342554No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.8097369612231554No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1580.79464869486496No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.7342956294321783No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.7342956294321783No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.7342956294321783No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1420.7141779409545843No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1410.7091485188351858No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.6840014082381934No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.6739425639993964No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1290.6487954534024041No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1280.6437660312830056No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1120.56329527737263No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.5381481667756375No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1020.5130010561786451No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA990.49791278982044956No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA970.4878539455816526No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA960.4828245234622542No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA950.4777951013428557No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA950.4777951013428557No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.47276567922345725No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA930.46773625710405875No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA910.45767741286526176No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA890.4476185686264648No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.4476185686264648No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA850.4275008801488709No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA800.40235376955187846No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA770.387265503193683No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA760.3822360810742846No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.36714781471608915No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA730.36714781471608915No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA700.3520595483578937No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA690.3470301262384952No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA680.3420007041190967No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA670.3369712819996982No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA660.33194185988029973No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA580.2917064829251119No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.2917064829251119No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.2917064829251119No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA560.281647638686315No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.281647638686315No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA550.27661821656691643No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.2665593723281195No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA520.261529950208721No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.25650052808932255No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA510.25650052808932255No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.25650052808932255No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA500.25147110596992406No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA490.2464416838505256No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA490.2464416838505256No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA480.2414122617311271No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA470.23638283961172862No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA470.23638283961172862No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA470.23638283961172862No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.23638283961172862No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA470.23638283961172862No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.23135341749233015No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA450.22632399537293166No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA450.22632399537293166No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA450.22632399537293166No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.2212945732535332No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.2212945732535332No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA440.2212945732535332No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA440.2212945732535332No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA430.21626515113413466No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA430.21626515113413466No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.21626515113413466No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.21626515113413466No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA420.2112357290147362No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA420.2112357290147362No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA410.2062063068953377No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA410.2062063068953377No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.2062063068953377No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA400.20117688477593923No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.20117688477593923No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA390.19614746265654076No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.19614746265654076No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA380.1911180405371423No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA370.1860886184177438No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA370.1860886184177438No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.1860886184177438No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.18105919629834533No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.18105919629834533No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.18105919629834533No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.17602977417894686No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.17100035205954836No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA340.17100035205954836No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA340.17100035205954836No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.17100035205954836No Hit
TCCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA320.1609415078207514No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.1609415078207514No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.15591208570135293No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA310.15591208570135293No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA300.15088266358195443No Hit
ACCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA300.15088266358195443No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.1408238193431575No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA280.1408238193431575No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.135794397223759No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.135794397223759No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA270.135794397223759No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA270.135794397223759No Hit
GCCTACGGGAGGCTGTAGTGGGGAATATTGCACAATGGGGGAAACCCTGA270.135794397223759No Hit
TCCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA260.1307649751043605No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGA260.1307649751043605No Hit
TCCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA260.1307649751043605No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA260.1307649751043605No Hit
GCCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA260.1307649751043605No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA250.12573555298496203No Hit
TCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA250.12573555298496203No Hit
CCCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA250.12573555298496203No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA250.12573555298496203No Hit
ACCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA250.12573555298496203No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA250.12573555298496203No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA250.12573555298496203No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA240.12070613086556355No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA240.12070613086556355No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA240.12070613086556355No Hit
GCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA240.12070613086556355No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.12070613086556355No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA230.11567670874616508No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA230.11567670874616508No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA230.11567670874616508No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA230.11567670874616508No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA230.11567670874616508No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA230.11567670874616508No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA230.11567670874616508No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA230.11567670874616508No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA220.1106472866267666No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA220.1106472866267666No Hit
TCCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA220.1106472866267666No Hit
ACCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA220.1106472866267666No Hit
ACCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA210.1056178645073681No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA210.1056178645073681No Hit
GCCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA210.1056178645073681No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA210.1056178645073681No Hit
CCCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA200.10058844238796961No Hit
CCCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA200.10058844238796961No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA200.10058844238796961No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.10058844238796961No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.10058844238796961No Hit
CCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA200.10058844238796961No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.10058844238796961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)