FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32656
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3931.2034541891229789No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3641.1146496815286624No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3591.0993385595296423No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3561.0901518863302304No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3240.9921607055365017No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3220.9860362567368938No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3130.9584762371386575No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2940.9002939735423812No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2910.891107300342969No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2800.857422831945125No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2730.8359872611464968No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2670.8176139147476728No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2330.7134982851543361No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2310.7073738363547281No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2150.6583782459578638No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2150.6583782459578638No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1970.6032582067613914No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1920.5879470847623713No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1850.5665115139637433No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1810.5542626163645272No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1780.5450759431651152No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1740.5328270455658991No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1720.526702596766291No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.514453699167075No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1660.5083292503674669No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1630.49914257716805494No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.47464478196962273No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1520.46545810877021065No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.4470847623713866No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.43789808917197454No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.43789808917197454No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.4287114159725625No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.4287114159725625No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.42564919157275843No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.4195247427731504No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.41340029397354233No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1330.40727584517393434No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1310.4011513963743263No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.3980891719745223No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.3980891719745223No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.3980891719745223No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.3980891719745223No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.39196472317491426No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.38890249877511024No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.3858402743753062No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.3858402743753062No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.37971582557569816No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.37971582557569816No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1230.37665360117589414No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.3674669279764821No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.3674669279764821No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1180.36134247917687407No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.349093581577658No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.349093581577658No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.346031357177854No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.346031357177854No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.34296913277804997No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.33990690837824594No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.33990690837824594No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.3368446839784419No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.33072023517883387No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.3276580107790299No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.3276580107790299No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.3276580107790299No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.32153356197942184No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.3184713375796179No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.3092846643802058No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.3092846643802058No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.3062224399804018No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.30316021558059775No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA980.3000979911807937No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA980.3000979911807937No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.29397354238118567No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.29397354238118567No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.29397354238118567No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA950.29091131798138165No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.28478686918177365No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.28478686918177365No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.2756001959823616No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.2756001959823616No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.2756001959823616No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.2694757471827536No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.26641352278294955No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA870.26641352278294955No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA860.2633512983831455No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA860.2633512983831455No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA850.2602890739833415No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.2572268495835375No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.24804017638412543No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA800.2449779519843214No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.2449779519843214No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.24191572758451738No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.24191572758451738No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.24191572758451738No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA790.24191572758451738No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.23579127878490935No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA770.23579127878490935No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.23579127878490935No Hit
TGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA740.22660460558549728No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.22660460558549728No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.22048015678588928No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.21741793238608526No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.21741793238608526No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA700.21435570798628126No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA700.21435570798628126No Hit
TGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA700.21435570798628126No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA700.21435570798628126No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA690.21129348358647723No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA680.2082312591866732No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA670.20516903478686918No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA670.20516903478686918No Hit
TGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA660.20210681038706516No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.19904458598726116No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.19904458598726116No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.19904458598726116No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA640.19598236158745713No Hit
TGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA620.18985791278784908No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA620.18985791278784908No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA600.18373346398824106No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA600.18373346398824106No Hit
AGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA580.17760901518863303No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA570.174546790788829No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA560.17148456638902498No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA550.16842234198922096No Hit
AGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA550.16842234198922096No Hit
AGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA530.16229789318961294No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA520.15923566878980894No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA510.1561734443900049No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA490.15004899559039686No Hit
CGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA470.1439245467907888No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA470.1439245467907888No Hit
AGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA460.1408623223909848No Hit
CGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA460.1408623223909848No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA460.1408623223909848No Hit
AGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA450.1378000979911808No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA450.1378000979911808No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA450.1378000979911808No Hit
TGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA440.1347378735913768No Hit
AGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA420.12861342479176874No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA420.12861342479176874No Hit
CGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA420.12861342479176874No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA410.1255512003919647No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA410.1255512003919647No Hit
CGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA390.11942675159235669No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA390.11942675159235669No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA390.11942675159235669No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA380.11636452719255266No Hit
TGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA380.11636452719255266No Hit
AGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA380.11636452719255266No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA360.11024007839294464No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA360.11024007839294464No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA350.10717785399314063No Hit
AGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA350.10717785399314063No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA350.10717785399314063No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA350.10717785399314063No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA350.10717785399314063No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA350.10717785399314063No Hit
AGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA340.1041156295933366No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA340.1041156295933366No Hit
TGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA330.10105340519353258No Hit
TGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA330.10105340519353258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)