FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22782
Sequences flagged as poor quality0
Sequence length321
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA13956.123255201474849No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4702.063032218418049No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3751.6460363444824864No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3591.5758054604512333No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3511.540690018435607No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3261.4309542621367748No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3061.3431656570977086No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2871.259766482310596No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2711.1895355982793434No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2651.1631990167676236No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2651.1631990167676236No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2581.1324730050039507No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2431.066631551224651No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2421.0622421209726978No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2391.0490738302168379No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2341.0271266789570714No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2291.005179527697305No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2240.9832323764375385No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2230.9788429461855852No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2230.9788429461855852No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2210.9700640856816786No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2190.961285225177772No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2180.9568957949258187No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2120.9305592134140988No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1980.8691071898867527No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1960.8603283293828461No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1950.8559388991308928No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1950.8559388991308928No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1830.8032657361074532No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1830.8032657361074532No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.7944868756035466No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1790.7857080150996401No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.7813185848476867No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1650.7242559915722939No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1550.680361689052761No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.6276885260293215No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1410.6189096655254147No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.6013519445176017No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1340.5881836537617418No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1300.5706259327539286No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.5530682117461154No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1230.5398999209902554No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1130.4960056184707225No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1120.49161618821876923No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1110.4872267579668159No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA1060.4652796067070494No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1040.4565007462031428No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA990.43455359494337636No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.40382758317970324No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA840.3687121411640769No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA800.35115442015626375No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA730.3204284083925907No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA680.29848125713282414No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA640.280923536125011No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA630.2765341058730577No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA630.2765341058730577No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA620.27214467562110434No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA610.2677552453691511No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA600.26336581511719775No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA600.26336581511719775No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA590.2589763848652445No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA580.25458695461329117No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA540.23702923360547798No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA540.23702923360547798No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.23702923360547798No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA520.2282503731015714No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA510.2238609428496181No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA500.21947151259766481No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.21508208234571152No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.21508208234571152No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA490.21508208234571152No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.21508208234571152No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA470.20630322184180494No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA460.20191379158985162No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.19313493108594507No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.19313493108594507No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA420.18435607058203846No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA420.18435607058203846No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA400.17557721007813187No Hit
ACCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA400.17557721007813187No Hit
CCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA400.17557721007813187No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA390.17118777982617855No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA390.17118777982617855No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA380.16679834957422526No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA370.16240891932227197No Hit
ACCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA370.16240891932227197No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA360.15801948907031868No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.15801948907031868No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA360.15801948907031868No Hit
CCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA360.15801948907031868No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA360.15801948907031868No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA360.15801948907031868No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.15363005881836536No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA350.15363005881836536No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.15363005881836536No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA350.15363005881836536No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.14924062856641207No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA340.14924062856641207No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA340.14924062856641207No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA330.14485119831445878No Hit
GCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA330.14485119831445878No Hit
GCCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA330.14485119831445878No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA320.1404617680625055No Hit
GCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA320.1404617680625055No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA310.13607233781055217No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.13607233781055217No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA310.13607233781055217No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA310.13607233781055217No Hit
ACCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA310.13607233781055217No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA300.13168290755859888No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA300.13168290755859888No Hit
GCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA290.12729347730664559No Hit
TCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA290.12729347730664559No Hit
ACCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA290.12729347730664559No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA290.12729347730664559No Hit
TCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA280.12290404705469231No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA280.12290404705469231No Hit
CCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA270.11851461680273899No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA270.11851461680273899No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA260.1141251865507857No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA260.1141251865507857No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA260.1141251865507857No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA260.1141251865507857No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA260.1141251865507857No Hit
TCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA260.1141251865507857No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA250.10973575629883241No Hit
ACCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA250.10973575629883241No Hit
CCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA250.10973575629883241No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGA250.10973575629883241No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA250.10973575629883241No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA250.10973575629883241No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA240.10534632604687912No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA240.10534632604687912No Hit
CCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA240.10534632604687912No Hit
GCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA240.10534632604687912No Hit
ACCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA240.10534632604687912No Hit
CCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA240.10534632604687912No Hit
TCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA240.10534632604687912No Hit
CCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA230.10095689579492581No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA230.10095689579492581No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA230.10095689579492581No Hit
TCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA230.10095689579492581No Hit
TCCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA230.10095689579492581No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA230.10095689579492581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)