FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23485
Sequences flagged as poor quality0
Sequence length281
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4271.8181818181818181No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4101.7457951884181395No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3951.6819246327443047No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3671.5626995954864806No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3521.4988290398126463No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3511.4945710027677241No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3251.3838620395997445No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3161.345539706195444No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2991.2731530764317651No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2971.2646370023419204No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2961.260378965296998No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2941.2518628912071534No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2841.2092825207579305No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2441.0389610389610389No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2391.0176708537364276No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2260.9623163721524377No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2210.9410261869278264No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2030.8643815201192251No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1930.8218011496700022No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1910.8132850755801576No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1790.76218863104109No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1790.76218863104109No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1750.7451564828614009No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.7366404087715563No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1700.7238662976367894No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.7153502235469449No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.7153502235469449No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1660.7068341494571003No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1650.702576112412178No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1620.6898020012774111No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1600.6812859271875665No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1570.6685118160527996No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1560.6642537790078774No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1540.6557377049180327No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1500.6387055567383436No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1500.6387055567383436No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1500.6387055567383436No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1490.6344475196934213No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.630189482648499No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.6259314456035767No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.6216734085586545No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.6088992974238876No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.6088992974238876No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.5961251862891207No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.5961251862891207No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.5961251862891207No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.5918671492441985No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1380.5876091121992761No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1360.5790930381094316No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1360.5790930381094316No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1320.5620608899297423No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.5407707047051309No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.5407707047051309No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.5279965935703641No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1230.5237385565254418No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1230.5237385565254418No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1210.5152224824355972No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1210.5152224824355972No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.5067064083457526No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1180.5024483713008303No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1150.48967426016606347No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.48541622312114113No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.48115818607621885No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.48115818607621885No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.47690014903129657No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.47690014903129657No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.468384074941452No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.468384074941452No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.468384074941452No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.468384074941452No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.4598680008516074No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.4598680008516074No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.4598680008516074No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.4598680008516074No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.45135192676176283No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.44709388971684055No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.44709388971684055No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1030.43857781562699594No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.43431977858207366No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.43431977858207366No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.4300617415371514No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.4300617415371514No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.4300617415371514No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.4258037044922291No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.4130295933574622No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.4130295933574622No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.4130295933574622No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.4087715563125399No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.4087715563125399No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.395997445177773No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.38748137108792846No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.3789652969980839No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.3789652969980839No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.37470725995316156No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA860.366191185863317No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA860.366191185863317No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.35767511177347244No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.32786885245901637No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.3193527783691718No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.3193527783691718No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.3150947413242495No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.29380455609963807No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.2767724079199489No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.25122418565041515No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA480.20438577815626996No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA410.1745795188418139No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA360.15328933361720248No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA330.1405152224824356No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA320.1362571854375133No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA310.13199914839259103No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA300.12774111134766872No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA300.12774111134766872No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA300.12774111134766872No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA270.11496700021290185No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA270.11496700021290185No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA260.11070896316797957No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA250.10645092612305727No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA250.10645092612305727No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA250.10645092612305727No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA240.10219288907813498No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA240.10219288907813498No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA240.10219288907813498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)