FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49852
Sequences flagged as poor quality0
Sequence length321
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA25225.058974564711546No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA10952.1965016448688117No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8761.7572013158950492No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7121.4282275535585331No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6991.4021503650806388No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5841.171467543930033No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5681.1393725427264703No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5351.0731766027441227No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5291.0611409772927867No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5231.0491053518414508No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4880.9788975367086576No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4530.9086897215758646No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4460.8946481585493059No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4420.8866244082484154No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4350.8725828452218566No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA4260.8545294070448527No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4220.8465056567439622No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4190.8404878440182941No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4160.8344700312926261No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4030.8083928428147317No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4020.806386905239509No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4000.8023750300890636No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3870.776297841611169No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3820.7662681537350557No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3660.7341731525314932No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3620.7261494022306026No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3600.7221375270801572No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA3580.718125651929712No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3570.7161197143544893No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3510.7040840889031533No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3460.6940544010270401No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3170.6358822113455829No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3160.6338762737703603No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA3070.6158228355933563No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA3000.6017812725667977No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2930.5877397095402391No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2890.5797159592393485No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2880.5777100216641258No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2880.5777100216641258No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2720.5456150204605633No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA2670.53558533258445No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA2630.5275615822835593No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2610.523549707133114No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2550.511514081681778No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA2500.5014843938056648No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2450.4914547059295515No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2450.4914547059295515No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.46738345502687956No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA2300.4613656423012116No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA2260.453341892000321No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA2220.44531814169943035No Hit
TCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA2210.4433122041242077No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA2180.43729439139853965No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA2110.4232528283719811No Hit
GCCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA2030.40720532777019974No Hit
TCCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1990.3991815774693092No Hit
TCCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1980.39717563989408644No Hit
GCCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1950.3911578271684185No Hit
GCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1930.3871459520179732No Hit
TCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1900.3811281392923052No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1880.3771162641418599No Hit
ACCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1860.37310438899141457No Hit
TCCTATGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1860.37310438899141457No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1860.37310438899141457No Hit
GCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1840.3690925138409693No Hit
TCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1820.36508063869052393No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGA1810.3630747011153013No Hit
ACCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1810.3630747011153013No Hit
GCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1770.3550509508144106No Hit
CCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1730.34702720051352004No Hit
CCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1710.3430153253630747No Hit
ACCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1650.33097969991173876No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1600.32095001203562545No Hit
CCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1600.32095001203562545No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1590.3189440744604028No Hit
ACCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1520.30490251143384417No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA1320.264783759929391No Hit
CCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1280.2567600096285004No Hit
CCCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1280.2567600096285004No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.24873625932760973No Hit
CCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1240.24873625932760973No Hit
CCCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1210.24271844660194172No Hit
CCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1190.23870657145149643No Hit
ACCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1180.23670063387627374No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1140.22867688357538313No Hit
TCCTACGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1130.2266709460001605No Hit
GCCTATGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1120.2246650084249378No Hit
ACCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1100.22065313327449249No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA1090.21864719569926983No Hit
GCCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1090.21864719569926983No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1080.2166412581240472No Hit
CCCTATGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1030.2066115702479339No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1030.2066115702479339No Hit
ACCTATGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1020.20460563267271126No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1020.20460563267271126No Hit
CCCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1010.20259969509748857No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA970.19457594479659793No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA950.1905640696461526No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA930.18655219449570729No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA930.18655219449570729No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA920.18454625692048465No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA900.1805343817700393No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA890.17852844419481664No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA890.17852844419481664No Hit
GCCTACGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA880.176522506619594No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA870.17451656904437135No Hit
ACCTACGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA860.17251063146914866No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA810.1624809435930354No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA800.16047500601781273No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA790.15846906844259007No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA780.1564631308673674No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA760.15245125571692208No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA760.15245125571692208No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA750.15044531814169942No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA750.15044531814169942No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA730.14643344299125413No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA730.14643344299125413No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA720.14442750541603144No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA710.14242156784080878No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA700.14041563026558612No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA690.1384096926903635No Hit
ACCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA690.1384096926903635No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA690.1384096926903635No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGA670.13439781753991814No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA650.13038594238947285No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.1283800048142502No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.12637406723902753No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA630.12637406723902753No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.12436812966380487No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.12436812966380487No Hit
GCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA610.1223621920885822No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.12035625451335955No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA600.12035625451335955No Hit
CCCTACGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA600.12035625451335955No Hit
GCCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA570.11433844178769156No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA560.1123325042124689No Hit
CCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA560.1123325042124689No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA550.11032656663724624No Hit
TCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA540.1083206290620236No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA540.1083206290620236No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA540.1083206290620236No Hit
GCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA530.10631469148680094No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA530.10631469148680094No Hit
GCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA530.10631469148680094No Hit
GCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA530.10631469148680094No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA520.10430875391157826No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.10430875391157826No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA510.10230281633635563No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGA510.10230281633635563No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGA500.10029687876113295No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.10029687876113295No Hit
TCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA500.10029687876113295No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.10029687876113295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)