FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008091963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008091963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25451
Sequences flagged as poor quality0
Sequence length321
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA17857.013476877136458No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5722.247455895642607No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4981.9567011119405917No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4221.6580880908412243No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4171.6384424973478449No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4091.6070095477584379No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3751.4734195120034577No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3701.4537739185100782No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3691.4498447998114024No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3591.4105536128246434No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3181.249459746178932No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3161.2416015087815802No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3111.221955915288201No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3051.1983812030961456No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3021.1865938470001178No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3001.1787356096027661No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2981.1708773722054142No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA2801.1001532356292483No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2791.0962241169305724No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2781.0922949982318966No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2731.072649404738517No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2721.0687202860398413No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2701.0608620486424893No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2701.0608620486424893No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2611.0254999803544065No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2551.0019252681623512No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2450.9626340811755923No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.9469176063808888No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2370.9312011315861852No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2300.903697300695454No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2060.8093984519272327No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2010.7897528584338533No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2010.7897528584338533No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1920.7543907901457703No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.6993831283643078No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.6993831283643078No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.695454009665632No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.660091941377549No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1600.6286589917881419No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1600.6286589917881419No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1580.6208007543907901No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.6011551608974107No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1320.518643668225217No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1270.4989980747318376No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1270.4989980747318376No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA1080.42434481945699576No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1020.40077010726494045No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA950.3732662763742092No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA900.3536206828808298No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA880.345762445483478No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA800.31432949589407094No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA770.3025421397980433No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA760.2986130210993674No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA750.29468390240069153No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA750.29468390240069153No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA730.2868256650033397No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA720.2828965463046639No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA690.27110919020863616No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA650.25539271541393266No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA640.25146359671525675No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.25146359671525675No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.24753447801658088No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA620.24360535931790497No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.2396762406192291No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.2396762406192291No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.2357471219205532No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.2357471219205532No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA580.22788888452320144No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.21217240972849788No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA540.21217240972849788No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.21217240972849788No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA530.208243291029822No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.208243291029822No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA510.20038505363247022No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA510.20038505363247022No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.20038505363247022No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.20038505363247022No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.20038505363247022No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.20038505363247022No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.19645593493379435No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA480.18859769753644257No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA480.18859769753644257No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA480.18859769753644257No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA470.1846685788377667No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.1846685788377667No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA470.1846685788377667No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.1846685788377667No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA450.1768103414404149No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA450.1768103414404149No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.1768103414404149No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA450.1768103414404149No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.172881222741739No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA440.172881222741739No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA440.172881222741739No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA440.172881222741739No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.172881222741739No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA420.16502298534438725No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.16502298534438725No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGA410.16109386664571135No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA410.16109386664571135No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.16109386664571135No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA400.15716474794703547No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA400.15716474794703547No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.15716474794703547No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA400.15716474794703547No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.1532356292483596No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA390.1532356292483596No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.1493065105496837No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.14537739185100781No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA360.14144827315233194No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA360.14144827315233194No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA350.13751915445365603No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA350.13751915445365603No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.13359003575498016No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.13359003575498016No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA340.13359003575498016No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA330.12966091705630425No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA330.12966091705630425No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA320.12573179835762838No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGA310.12180267965895249No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA310.12180267965895249No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA300.1178735609602766No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA300.1178735609602766No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA300.1178735609602766No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA290.11394444226160072No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA290.11394444226160072No Hit
GCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA290.11394444226160072No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGGAACCCTGA290.11394444226160072No Hit
ACCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA280.11001532356292483No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.11001532356292483No Hit
TCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA280.11001532356292483No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA270.10608620486424894No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA270.10608620486424894No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.10608620486424894No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.10608620486424894No Hit
GCCTACGGGACGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA270.10608620486424894No Hit
GCCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA260.10215708616557306No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA260.10215708616557306No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA260.10215708616557306No Hit
TCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA260.10215708616557306No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA260.10215708616557306No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA260.10215708616557306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)